飽和脈衝葉綠素螢光影像系統之建立 及於植物光熱逆境生理之應用

Development of Saturation Pulse Fluorescence Imaging System and Its Applications to Plants under Light-Heat Stress

謝騄璘(Lu-Lin Hsieh)

指導教授 ： 陳世銘

國立臺灣大學/生物資源暨農學院/生物產業機電工程學研究所/碩士(2006年)

https://doi.org/10.6342/NTU.2006.02327

摘要

本研究開發了飽和脈衝螢光影像系統，以進行植物光熱逆境之生理狀態分析。系統中的光平面系統是藉由可程式邏輯控制器，依飽和脈衝葉綠素螢光量測方式所需要的弱調控光、光化學能光及飽和脈衝光三種不同強度光源，進行時間序列之控制。
影像擷取平台則搭配不同螢光激發狀態，進行攝影機訊號增益倍率之調控，以解決攝影機動態範圍之能力不足問題，再藉由影像擷取頻率之自動調變，增加螢光劇烈變化階段之時間解析能力，以及減少在螢光反應趨於穩定時之取樣速度；並於取像時一併記錄擷取影像之時間，再以自行開發之螢光影像處理程序，分析完整之螢光影像資訊。
當植物處於正常狀態和不同光熱逆境階段時，經由本系統分析所得之『葉綠素螢光非光化學淬熄指標』、『葉綠素螢光非光化學淬熄指標變化曲線』和『螢光指標強度影像』等三種螢光指標，相較於市售MINI-PAM之量測結果，驗證本系統具備檢測植物逆境生理變化之能力。
此外，因本系統可以獲得完整葉面之螢光反應，考慮葉面上各部位因逆境所產生之變異，與市售單點儀器比較後，本系統量測逆境植物有更為一致之量測結果。飽和脈衝葉綠素螢光影像系統提供另一種途徑，可讓研究者以非破壞性的方式探討植物生理現象。

關鍵字

螢光影像 ； 植物逆境 ； 飽和脈衝法 ； 光合作用

並列摘要

In this article, the saturation pulse fluorescence imaging system (SPFIS) was developed to analyze to physical status of plants under light and heat stress. One part of SPFIS, the light plane system, was controlled by a programmable logic controller (PLC) to supply three different lighting conditions, including weak modulated measuring beam, continuous actinic light and saturating pulse lights, during measuring period.
The other part of SPFIS, fluorescence imaging system, was developed to grab images with different signal gain setting of the CCD camera according to the different lighting conditions. The function of gain setting was used to overcome the insufficiency of camera dynamic range. Besides, the imaging frequency of SPFIS was auto adjusted to increase the time resolution when the fluorescence reaction change quickly and to decrease the sampling rate when the fluorescence reaction is stable. The imaging time was also recorded. The serial fluorescence images and the time record of plants were computed by the designed image processes to extract the complete fluorescence image information.
The plants, under normal and different phases of light and heat stress, were analyzed by SPFIS to obtain three fluorescence results, including non-photochemical quenching of chlorophyll fluorescence, non-photochemical quenching curve of chlorophyll fluorescence, and intensity image of fluorescence index. Compared with the measurement results of the same plant by mini-PAM, SPFIS had the ability to detect the variation of physical status when plants were under stress.
Moreover, according to SPFIS could obtain the complete fluorescence reaction of full leaves and consider the variations of full leaves under stress, the measurement results showed more consistent than mini-PAM, when plants were under stress condition. Saturation pulse fluorescence imaging system provides another way for research workers to monitoring plant physiological in non-destroy style.

並列關鍵字

Fluorescence imaging ； Plant stress ； Saturation Pulse Method ； Photosynthesis

參考文獻

林怡如、葉德銘。2004。出瓶馴化光度與培植期對’綠巨人’白鶴芋組培苗出瓶生長之影響。中國園藝50(1):21-30。

陳世銘、馮丁樹、洪慎德、吳春杰、呂昆忠、田秉才、張文宏。1996。柑橘類水果光電選別機之研製。農業機械學刊5(4):35-46。

陳金男。2005。LED光環境控制系統之建立與其應用於星辰花組培苗生理影響之探討。碩士論文。台北：國立臺灣大學生物產業機電工程學研究所。

張智星。2000。MATLAB程式與應用。新竹：清蔚科技股份有限公司。

呂宏志。2005。溫室多功能監測系統之開發 : 苗床植株遥測與環境因子量測。碩士論文。台北：國立台灣大學生物產業機電工程學研究所。