基於簡單重複序列標記的台灣主要稉稻品種鑑別技術

Variety Identification among Major Japonica Rice Cultivars of Taiwan Based on Simple Sequence Repeat Markers

謝廉一(Lien-Yi Hsieh)

指導教授 ： 胡凱康

國立臺灣大學/生物資源暨農學院/農藝學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.01809

摘要

為鑑別台灣地區的主要稉稻品種，本研究利用46對簡單重複序列引子對25個參試品種(系)進行分析。在經過聚合酵素連鎖反應及螢光標記自動偵測系統記錄之後，其中的20組引子在參試品種之中具有多型性，平均多型性數為2.90；PIC值為0.383。除了台稉11號及台稉15號的分子標記多型性模式完全相同之外，其餘品種(系)均可以完全被鑑別。除此之外，對所有參試品種(系)及現有栽培品種中計算出鑑別最小組合引子對分別是6個與4個。而對於現有栽培品種中，也設計出一組可以進行的Multiplex PCR的SSR引子對組合。

關鍵字

簡單重複序列 ； 品種鑑別 ； 稻 ； 稉

並列摘要

In order to identify the major Japonica type rice cultivars grown in Taiwan, this research used 46 pairs of simple sequence repeat (SSR) primers conducting analysis on 25 rice cultivars. After polymerase chain reaction (PCR) and automatic DNA fragment detection based on primer labeled with florescent dye, 20 pairs of primers showed polymorphism among cultivars. In total, there were 21 polymorphic loci, with average 2.9 alleles per locus, averaged Polymorphic Information Content is 0.383. All cultivars can be distinguished based on polymorphic SSR markers, except Taikeng No. 11 and Taikeng No. 15. The minimum set of primers that can be used to distinguish all 25 entries and 18 major cultivars are composed of 6 and 4 pairs or primers, respectively. For distinguishing major cultivars, a set of 4 pairs of primers, which are suitable for Multiplex PCR, is also found.

並列關鍵字

Multiplex PCR ； simple sequence repeat (SSR) ； Japonica rice ； variety identification

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