高密度發酵菌種篩選

Screening of high density fermentor yeast strains

朱傳大(Chuan-TA Chu)

指導教授 ： 陳銘凱

淡江大學/理學院/化學學系碩士班/碩士(2019年)

https://doi.org/10.6846/TKU.2019.00935

摘要

酒精不管做為能源還是食物一直以來是人類依賴的重要產物，如何產酒以及增加產酒效率一直以來是工業中的課題之一。
在過往的研究中，學家利用Methylnitronitrosoguanidine(MNNG)或Ethyl Methane Sulfonate(EMS)藥劑與UV突變法對酵母菌BY4742菌株進行基因突變，更有利用Global transcription machinery engineering （gTME）基因工程的方式轉換細胞基因，期望利用改變原有基因配置能增加酵母菌在高醣高酒精的環境下酒精生產的效率。
本實驗採UV突變法並以3000、4000、5000μJ/cm2強度進行照射突變，再藉由高濃度葡萄糖與高濃度酒精的培養條件，對已隨機突變過的菌株進行連續液態篩選，初步篩選出具有高醣高酒精耐受性的菌種。

關鍵字

酵母菌BY4742 ； 酒精發酵 ； 突變 ； 酒精耐受性

並列摘要

Alcohol has always been an important product of human dependence,
whether it is energy or food. How to produce wine and increase the efficiency of
wine production has always been one of the topics in the industry.
In previous studies, scientists used Methylnitronitrosoguanidine (MNNG)
or Ethyl Methane Sulfonate (EMS) agents and UV mutations to genetically
mutate the yeast BY4742 strain, furthermore, converted the genes of the cells by
using Global transcription machinery engineering (gTME), expected to use the
altered original gene configuration that can increase the efficiency of yeast
production in high concentration of glucose and alcohol environments.
In our experiment, UV mutation method was used to irradiate mutations
with intensity of 3000, 4000 and 5000 μJ/cm2, and the strains with random
mutations were subjected to continuous liquid screening by high-concentration
glucose and high-concentration alcohol culture conditions. In order to find a
strain with high sugar and high alcohol tolerance.

並列關鍵字

Saccharomyces cerevisiae BY4742 ； alcohol fermentation ； mutation ； ethanol tolerance

參考文獻

1. McGovern, P.E., et al., Fermented beverages of pre- and proto-historic China, Proceedings of the National Academy of Sciences, 2004. 101(51), P. 17593-17598.

2. G. M. Walker, 125th Anniversary Review: Fuel Alcohol: Current Production and Future Challenges., J. Inst. Brew, 2011.117(1), P.3–22.

3. Li, p., Sakuragi, k., Makino, H., Extraction techniques in sustainable biofuel production: A concise review, Fuel Processing Technology, 2019,193, P. 295-303.

4. Gray K.A., Zhao L., Emptage M., Current Opinion in Chemical Biology , Bioethanol, 2006. 10, P.141-146.

5. Antoni, D., Zverlov, V., Schwarz, W., Biofuels from microbes, Applied Microbiology and Biotechnology, 2007. 77, P. 23-35.