生質丁醇生產潛力菌株之篩選

Potential Bacterial Strains for Biobutanol Production

陳勁中(Chin-Chung Chen) ； 蔡承佳(Zheng-Chia Tsai) ； 陳寶東(Bao-Dong Chen)

《石油季刊》 47卷4期 (2011/12) Pp. 85-98

https://doi.org/10.30128/JP.201112.0007

摘要

使用丁醇生產菌種（Clostridium）進行ABE發酵作用，是生產生質丁醇最主要的方法。生質丁醇的製造成本是發展生質丁醇工業所需面對最重大的難題。要達到降低製造成本最根本的方式，就是得到一株高效能的丁醇生產菌種。自1930年代後期，台灣中油股份有限公司煉製研究所保存具潛力之高效能丁醇生產菌種至今。在本研究中，將此些菌種進行活化並篩選具高效能丁醇生產潛力的菌種，成功挑選出六株菌種並進行菌種鑑定，四株菌種為Clostridium，另外兩株菌種為Staphylococcus，將其中丁醇生產效率最高的Clostridium菌種命名為Clostridium acetobutylicum CPCNE。利用批次式搖瓶，對其進行不同濃度葡萄糖（碳源）饋料之小量發酵測試，發現6％葡萄糖最適於用以生產丁醇，經過72小時發酵作用後，此菌株可以產生18.6 g／L的溶劑。

關鍵字

生質丁醇 ； ABE發酵 ； 批次式

並列摘要

Nowadays ABE fermentation of Clostridium is the main process to produce biobutanol. The key problem associated with the industrial bioproduction of butanol is the cost of substrate. This problem can be overcome by using hyper-butanol-producing strains of Clostridium. The potential hyper-butanol-producing strains of Clostridium have been stored in Refining and Manufacturing Research Institute of CPC Corporation in Taiwan since the late 1930s. In this study, these strains were screened for the highest butanol-producing ones. Six great butanol-producing strains were identified to be activated. Four of them were Clostridium while the other two were Staphylococcus. The highest butanol-producing Clostridium, named as Clostridium acetobutylicum CPCNE, was fermented with different concentrations of glucose (carbon source) in batch-scale flasks and 6% glucose was found to be best feasible for the butanol production while the solvent produced by this strain was 18.6 g/L following 72 h of fermentation.

並列關鍵字

Biobutanol ； ABE Fermentation ； Batch-scale