Albendazole與龍膽瀉肝湯混合治療在小白鼠感染廣東住 血線蟲誘發寄生蟲性腦膜炎之療效

Efficacy of albendazole and Long-dan-xie-gan-tan co-therapy against Angiostrongylus cantonensis induced parasitic meningitis in BALB/c mice

張永輝(Yung-Huei Chang)

指導教授 ： 李秀雄

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2005年)

https://doi.org/10.6834/CSMU.2005.00026

摘要

廣東住血線蟲症感染腦部，引起嗜伊紅性腦膜炎與腦膜腦炎時，albendazole是優先被考慮用來作驅蟲藥，但是被殺死的蟲體堆積在腦部時，再次引發嚴重的免疫反應，可能造成腦部更大的傷害。龍膽瀉肝湯是一種普遍在中醫方劑中，應用範圍廣泛的抗發炎方劑。本實驗分析一些發炎治療指標，包括 1) 腦脊髓液(cerebrospinal fluid，CSF)的組織型血纖維蛋白酶原( tissue type plasminogen activator, t-PA)、尿激酶型血纖維蛋白酶原（urokinase type plasminogen activator, u-PA）及基質金屬蛋白酶-9 (Matrix metalloproteinases-9, MMP-9)生化檢查 2) 用Haematoxylin-eosin staining之病理組織切片來看腦膜炎的組織變化 3) 再以線蟲蟲體回收數量是否降低。實驗結果顯示廣東住血線蟲所引發的腦膜炎與腦膜腦炎，用albendazole與龍膽瀉肝湯共同治療能降低發炎指標 tPA、uPA、and MMP-9;的活性，也會減少腦脊髓液中蟲體回收數量與嗜伊紅白血球，所以龍膽瀉肝湯在治療蛛蜘膜下腔的發炎反應中扮有抗發炎的角色。

關鍵字

龍膽瀉肝湯 ； 廣東住血線蟲 ； 嗜伊紅性腦膜炎

並列摘要

The anthelmintic agent albendzole can kill the Angiostrongylus cantonensis larva the infect the brain, the dead larvae are capable of evoking a severe, brain damaging immune response. Long-dan-xia-gan-tan(LDXGT) is one of the most common herbal medicines used to ant-inflammatory reaction . Assay indicators for the therapeutic effect include: 1) CSF biochemical test, such as tPA, uPA, and MMP-9, 2) histopathological score of meningitis, and 3) worm recovery. The result show that the albendazole- LDXGT co-therapy significantly decreased (P<0.05) the activity of tPA, uPA, and MMP-9, CSF eosinophil and worm recovery . These finding indicate that albendazole- LDXGT co-therapy exerted significant effects on parasitic meningitis. Conclusively, this herbal drug may have theraputical role in inflammatory reaction of subarachid space。

並列關鍵字

Long-dan-xie-gan-tan ； Angiostrongylus cantonensis ； eosinophilic meningitis

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