水飛薊素在四氯化碳誘發大鼠肝纖維化的療效

Therapeutic effects of silymarin on liver fibrosis induced by carbon tetrachloride (CCl4) in the Rat

何佩珍(Pei-Chen Ho)

指導教授 ： 劉哲育

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2005年)

https://doi.org/10.6834/CSMU.2005.00090

摘要

水飛薊素（silymarin）是水飛薊種子萃取物，它被當作草藥已有久遠歷史，在亞洲及歐洲水飛薊素臨床上被用作保肝藥品也有多年歷史，本研究以20﹪四氯化碳誘導大鼠肝纖維化動物模式探討其抑制纖維化療效。本實驗共分為正常組、負控制組（20﹪四氯化碳 0.25ml/100gm）、實驗組（silymarin 0.2g/ml），20﹪四氯化碳誘導八週後從第八週起每週管餵水飛薊素四次，連續三週，藉由水飛薊素在四氯化碳誘導大鼠肝纖維化上生化數值及組織學上改變，肝纖維化因子α-SMA及MMP-2之表現，評估其對肝纖維化療效。實驗結果發現餵食水飛薊素AST、ALT、Alkaline phosphatase（304±15.6、102±12.1U/L、496±77.2mg/dl）低於負控制組（349±7.6、125±19 U/L、622±36.7 mg/dl），而組織切片也顯示出餵食水飛薊素可以降低膠原纖維增生，而在肝臟器官相對重量（3.83 ± 0.16g）則餵食水飛薊素較四氯化碳組（4.04 ± 0.22g）為低，另外在四氯化碳誘發肝纖維化中水飛薊素可以抑制α-SMA蛋白活性及MMP-2活性之表現量。綜合以上結果推論在四氯化碳誘導大鼠肝纖維化模式，水飛薊素對於抑制肝纖維化形成具有療效。

關鍵字

水飛薊素 ； 肝纖維化

並列摘要

Silymarin, a standardized extract of the milk thistle（Silybum marianum） has a long tradition as an herbal remedy. The flavonoid silymarin was introduced as a“hepatoprotective”agent a few years ago and is used clinically in Europe and Asia for the treatment of liver diseases. In this study, we investigate the antifibrotic effect of the Silymarin on 20%CCl4（0.25ml/100gm） induced hepatic fibrosis in the rats.There were divided into three group, including normal control group（not treated with CCl4 ）,negative group to be only treated with CCl4 for 8 weeks and experimental group to be treated with Silymarin 3 weeks after the last of 8 weeks CCl4 administration.To evaluated therapeutic effects of silymarin by biochemical and histological alterations，we determination the results show that experimental group of silymarin treatment can significantly decreased the content of AST、ALT、ALP（p＜0.05），and liver weight/body weight ratio （p＜0.05）.more over the expression of α-SMA and MMP-2 were also significantly decreased in CCl4-induced liver fibrosis.These results suggested that Silymarin may have therapeutic effects in CCl4-induced liver fibrosis .

並列關鍵字

silymarin ； liver fibrosis ； therapeutic

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