蘆薈葉肉對於四氯化碳誘發老鼠肝損傷之影響

Effects of the Aloe Flesh on Carbon Tetrachloride-induced Liver Injury in Rats

吳東旭(Tung-Shine Wu)

指導教授 ： 劉珍芳

臺北醫學大學/公共衛生暨營養學院/保健營養學研究所/碩士(2013年)

摘要

慢性肝病及肝硬化為國人101年十大死亡原因之第九位，造成肝臟疾病主因有病毒性、酒精性與化學性三大類；其中化學性肝損傷於動物試驗模式之病理切片與人體有一致之病理現象。本試驗係依照行政院衛生署公告之健康食品之護肝功效評估方法（針對化學性肝損傷），探討庫拉索蘆薈葉肉對四氯化碳（carbon tetrachloride,CCl4）所誘導之Sprague-Dawley (SD) 大鼠肝臟慢性損傷的影響。試驗物質為冷凍乾燥後的蘆薈葉肉粉末，試驗動物共分為六組:正常控制組、正負對照組與低、中、高劑量試驗組 （200, 600, 1200 mg/kg）。正常控制組給予Olive oil，正對照組給予Silymarin，負對照組未給予任何物質；除正常控制組外，五個組別每週兩次以管餵給予CCl4持續8週。結果顯示低劑量試驗組對CCl4誘發慢性肝炎的血漿ALT、AST有顯著降低作用。高劑量試驗組對肝臟纖維化的指標，例如肝纖維化病理評分結果、肝臟中hydroxyproline含量皆有明顯降低作用。此外三組劑量組皆能提升大鼠肝臟中catalase, glutathione peroxidase 以及glutathione reductase等抗氧化酵素之含量，但不能提升glutathione與superoxidase dismutase含量。由上述結果顯示，給予大鼠凍乾蘆薈葉肉粉末，可經由增加肝臟抗氧化酵素含量、減少肝組織纖維化發生以及降低血漿AST、ALT值，達到減輕CCl4所誘發之大鼠肝臟慢性損傷的效果。

關鍵字

蘆薈、四氯化碳、化學性肝損傷

並列摘要

Chronic liver disease and cirrhosis is the eighth leading cause of death in 2012 in Taiwan.The main factors causing liver disease are virus, alcohol and chemicals ; including chemical liver injury model in animal experiments and human tissue sections there are consistent pathological phenomenon. The study was in accordance with the Department of Health announcement hepatoprotective effect of health food assessment methods (for chemical liver injury), to investigate the effect of Aloe vera leaf on carbon tetrachloride (carbon tetrachloride, CCl4)- induced chronic liver injury in Sprague-Dawley (SD) rats. Test substance was freeze dried aloe leaf powder. The test animals were divided into six groups: normal control group, positive and negative control group, the test group have three groups: low, medium and high dose treatment group (200,600,1200 mg / kg). Normal control group was given Olive oil, the positive control group was given silymarin 200 mg / kg, the negative control group did not give any substance; besides to the normal control group, the five groups twice a week to give CCl4 tube feeding for 8 weeks. The results showed that low-dose test group for chronic hepatitis induced by CCl4 elevated plasma AST, ALT value was significantly inhibited. Low-dose test group CCl4-induced liver fibrosis indicators, such as serum albumin concentration, the relative weight of the spleen, liver collagen, hydroxyproline content was significantly reduced. On histopathology of liver fibrosis score also showed the same result. Low, medium and high dose groups had encountered three upgrade rat liver catalase, glutathione peroxidase antioxidant enzyme content, but could not raise glutathione levels. The above results show that freeze-dried aloe leaf powder given, via increasing levels of antioxidant enzymes, reducing hepatic fibrosis and decreasing plasma AST, ALT values, to reduce the CCl4-induced chronic liver injury.

並列關鍵字

Aloe, carbon tetrachloride, chemical liver injury

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