樟芝固態發酵物抗四氯化碳誘發小鼠急慢性肝損傷之保肝活性及其品質評價

Solid fermented Antrodia cinnamomea inhibit acute and chronic hepatic injury induced by carbon tetrachloride (CCl4) and quality assessment.

曾承煒(Cheng-Wei Tzeng)

指導教授 ： 顏銘宏

高雄醫學大學/藥學院/天然藥物研究所碩士班/碩士(2008年)

摘要

樟芝 (Antrodia cinnamomea)在台灣是一著名的民間藥物，屬台灣特有種真菌，只生長在台灣特有的牛樟樹木材上，且無法人工培養。樟芝具有多種的生理功效，包括抗氧化、抗癌及肝臟保護作用。先前之研究發現樟芝具有抗B型肝炎病毒之活性。本研究的目的是開發樟芝的藥物資源並評估樟芝固態發酵物及其酒精和熱水萃取物對四氯化碳所誘發之小鼠急性及慢性肝損傷之肝臟保護活性。在急性(進行三天實驗)肝損傷實驗方面：以胃管餵食樟芝固態發酵物原物料懸浮液及酒精和熱水萃取物溶液後，由血液生化檢查顯示血清中GOT、GPT及LDH的濃度與四氯化碳組比較均有明顯降低的情形；藥物組在實驗中顯現了明顯抑制因腹腔注射四氯化碳所產生的三氯化碳自由基 (CCl3．)對肝臟造成傷害所產生的脂質過氧化的情形，而且隨著藥物濃度的增加抑制效果也越明顯。在慢性(進行八週實驗)實驗方面：樟芝固態發酵物組可以明顯的降低血中GOT、GPT及LDH值，且肝臟脂質過氧化的情形也會被抑制，而熱水及酒精萃取物則可降低血中LDH的濃度。由以上實驗結果推測樟芝固態發酵物可能經由抗氧化及清除自由基而抑制體內急性及慢性化學性肝損傷的作用，而具保肝活性。

關鍵字

樟芝固態發酵物 ； 抗肝炎 ； 抗氧化 ； 脂質過氧化 ； 肝臟保護活性

並列摘要

Antrodia cinnamomea, an endemic fungus in Taiwan, is well-known as a folk medicine, it grows only on the inner heartwood wall of the endemic evergreen Cinnamonum kanehirai and can not be cultivated. It has much physiological activities, including antioxidant, anticancer and hepatoprotective activity, and has active constituents that have anti-hepatitis B virus activity. The purpose of this research is to evaluate the hepatic protective effect of solid fermented A. cinnamomea against carbon tetrachloride (CCl4)-induced acute and chronic hepatic injury in vivo. In the acute liver injury experiment (go for three days)：after oral administration of solid fermented A. cinnamomea raw material suspension and ethanol extract and hot water extract, respectively, the results showed a significant decrease of GOT, GPT and LDH levels in mice serum compare to the carbon tetrachloride group. The raw material group showed the significant inhibition of liver lipid peroxidation induced by the carbon trichloride radical. In the chronic liver injury experiment (go for eight weeks)：raw material of solid fermented A. cinnamomea significantly decreased the level of serum GOT, GPT and LDH, and also inhibit liver lipid peroxidation. Ethanol and water extracts of solid fermented A. cinnamomea can decrease the blood LDH level significantly. These results demonstrated that solid fermented A. cinnamomea may exist hepatoprotective effect against acute and chronic chemical-induced heapatic injury in vivo, by mediating antioxidative and free radical scavenging activities.

並列關鍵字

Solid fermented Antrodia cinnamomea ； anti-hepatitis ； Antioxidant ； Lipid peroxidation ； Hepatoprotective activity

參考文獻

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