以回應曲面法探討水包油型奈米乳液最適化條件之研究

Study of optimization of oil in water nano-emulsions using response surface methodology

鄭叡駿(Ruei-Jiun Jeng)

指導教授 ： 陳文章

國立臺北科技大學/工程學院/化學工程研究所/碩士(2011年)

摘要

奈米乳液是由水相、油相以及界面活性劑三相所組成的乳化系統，粒徑分布通常為10~300奈米。由於其粒徑小，外觀上呈現透明或半透明，從熱力學的角度來看，為非平衡狀態，但在動力學上是穩定的狀態。
本研究以轉換乳液相 (Emulsion Inversion Point，EIP) 的方法，為一種低能量的乳化方式，將水相於固定流速下緩慢加入預先互溶的界面活性劑及油相以製備水包油型奈米乳液，界面活性劑選用PEG-40氫化蓖麻油和Span 80 兩種混合，油相則選用肉豆蔻酸異丙酯。利用回應曲面法(Respond Surface Methodology，RSM)進行實驗設計以探討各因子：PEG-40的含量、油與界面活性劑比、水的含量、溫度以及水的流速，對奈米乳液之粒徑的影響。實驗結果顯示，油與界面活性劑比例為最主要的影響因子，經由統計軟體之模擬預測在PEG-40含量為93.45%、油與界面活性劑比例為0.23、水含量為75.8%、溫度為38.7℃與水的流速為0.33mL/min的乳化製程條件下，可得到奈米乳液粒徑之預測值為58±0.1 nm，而實際以此最適化條件之實驗值可得到奈米乳液之粒徑為66.6±0.4 nm。

關鍵字

奈米乳液 ； 轉換乳液相 ； 水包油型乳液 ； 回應曲面法 ； 實驗設計

並列摘要

Nano-sized emulsions are composed of water phase, oil phase and surfactant mixture in an emulsified system with droplets ranging in size from 10 nm to 300 nm. Because of small-sized droplets they look like transparent or translucent. From a thermodynamic point of view, they are in the non-equilibrium state; however, they are also kinetically stable.
This study,investigates the nano-emulsfication methods involving the emulsion inversion point ( EIP ), which is a low-energy emulsification method. Oil in water emulsions were prepared by continuous addition of water with fixed flow rate to the oil phase containing surfactant mixture previously mixed together. The surfactant mixture is composed of PEG-40 hydrogenated and Span 80, and the oil phase is isoproply myristate. The particle size and distribution of nano-emulsions were investigated using response surface methodology (RSM) for design of experiments as a function of each factor: %PEG-40 content, oil-to-surfactant ratio, %water content, the temperature, and the flow rate of water. The experimental results showed that the oil-to-surfactant ratio is the most significant factor influencing the particle sizes of oil in water emulsions. Simulation by computer software predicted the particle size of 58±0.1 nm when optimal emulsification conditions were: %PEG-40 = 93.45%, oil-to- surfactant ratio =0.23, %water=75.8%﹐the temperature=38.7℃, and the flow rate of water = 0.33 mL/min, while the actual experimental value of particle size was obtained as 66.6±0.4 nm.

並列關鍵字

Nano-emulsions ； Emulsion inversion point ； Oil in water emulsions ； Response surface methodology ； Design of experiments

參考文獻

3.K. Shinoda, H. Saito, J. Colloid Interface Sci. 26 (1968) 70.

4.L. Marshall, Nonionic surfactants, in: M.J. Shick (Ed.), in: Surfactant Sciences Series, vol. 23, Marcel Dekker, New York, (1987) p. 493–547.

5.N. Anton ,T. F. Vandamme. Nano-emulsions and Micro-emulsions: Clarifications of the Critical Differences, Pharm Res 28 (2011) p. 978-985.

7.N. Anton, T. Vandamme, The universality of low-energy nanoemulsification.

Int J Pharm. 377 (2009)142–7.