以矽奈米線場效應電晶體偵測大腸桿菌F1-ATPase之交互作用

Detection of E.coli F1-ATPase interaction by silicon nanowire field effect transistors

陳霈樺(Pei-Hua Chen)

指導教授 ： 蔡麗珠

國立臺北科技大學/工程學院/有機高分子研究所/碩士(2014年)

摘要

F1-ATPase是一種膜蛋白，分別由三個α次單體(subunits)、三個β次單體與一個γ次單體所組合而成，且F1-ATPase可將ATP(Adenosine triphosphate)水解成ADP(Adenosine triphosphate)與磷酸(Pi)的活性功能。以基因重組的技術。將大腸桿菌的F1-ATPase α、β與γ次單體分別表現出來。再將單獨的α次單體與β次單體以重新重組的方式將α與β次單體組合成α3β3 complex，我們將α3β3 complex以化學修飾於矽線表面上，並利用矽奈米線場效應電晶體來觀察F1-ATPase與受質ATP的結合。實驗結果顯示，在β次單體或α3β3複合體中，加入 ATP會造成p型矽奈米線場效應電晶體的電流下降，表示ATP可以與β次單體或α3β3複合體結合。但實驗亦發現加入ATP進入修飾α次單體的奈米線不會造成電流的變化，證明ATP不會與α次單體反應。

關鍵字

F1-ATPase ； 矽奈米線場效應電晶體 ； 三磷酸腺苷

並列摘要

F1-ATPase is a kind of membrane protein. It is composed of three α and three β and one γ subunits. F1-ATPase could hydrolyze ATP to ADP and phosphate. In this study, we cloned and expressed individually the recombinant of α, β and γ subunits of E.coli F1-ATPase. After purification, equal amounts of α and β subunits were mixed to obtain α3β3 complex in vitro. The α and β subunits and α3β3 complex were then modified on the surface of silicon nanowire field effect transistors (SiNW-FETs). Introduction of ATP into p-type SiNW-FET modified with β subunit and α3β3 complex resulted in a decrease in the nanowire current, indicating reaction of ATP with β subunit and α3β3 complex. Contrarily, it is found that adding ATP into SiNW-FETs did not cause device current to change, suggesting that ATP does not react with α subunit.

並列關鍵字

F1-ATPase ； silicon nanowire field-effect-transistors ； Adenosine triphosphate

參考文獻

1. Chu, C.-C., et al., Improving Nanowire Sensing Capability by Electrical Field Alignment of Surface Probing Molecules. Nano Lett., 2013. 13: p. 2564-2569.

2. Hahm, J.-I. and C.M. Lieber, Direct Ultrasensitive Electrical Detection of DNA and DNA Sequence Variations Using Nanowire Nanosensors. Nano Lett., 2004. 4: p. 51-54.

3. patolsky, F., et al., Electrical detection of single viruses. PNAS, 2004. 101: p. 14017-14022.

4. Zeng, G., F. Patolsky, and C.M. Lieber, Multiplexed electrical detection of cancer markers with nanowire sensor arrays. NAT BIOTECHNOL 2005. 23: p. 1294-1301.

5. Patolsky, F., et al., Detection, stimulation, and inhibition of neuronal signals with high-density nanowire transistor arrays. Science, 2007. 5790: p. 1100-1104.