生物分子的交互作用對於人工關節材料摩擦行為之影響

Effects of Biomolecular Interactions on the Tribological Performance of artificial joint materials

賴宥勳(Yu-Hsun Lai)

指導教授 ： 方旭偉

國立臺北科技大學/工程學院/化學工程研究所/碩士(2009年)

摘要

超高分子量聚乙烯與鈷鉻鉬合金組合是最常見的人工關節植入物，而超高分子量聚乙烯的磨耗顆粒會引起骨溶解反應(osteolysis)，使得人工關節鬆脫，因而造成人工關節植入失敗。而人工關節之間有關節液的存在可以幫助關節間潤滑，減少關節元件的摩擦與磨耗。因此，不同關節液分子在潤滑時所扮演的角色為何？多成份關節液分子之間的交互作用，是否會造成不同的潤滑效果。所以關節液分子在生物摩擦系統中所扮演的角色，是值得我們更深入的研究討論。
本研究以關節液分子白蛋白(albumin)、球蛋白(globulin)、玻尿酸(hyaluronic acid)和磷脂質(phospholipids)，依其生理濃度配成仿生潤滑液(simulated synovial fluid)，之後再增加單成分關節液分子，探討各成分濃度的增加對於摩擦的影響。實驗結果指出，玻尿酸濃度為4.5 mg/mL時其摩擦係數為最低，此可能是因為玻尿酸能適當的補充潤滑液的黏度，有效幫助人工關節潤滑。之後我們進一步以玻尿酸搭配不同濃度的關節液分子，了解雙成份關節液分子間的交互作用對於人工關節潤滑機制的影響。從實驗結果發現，玻尿酸的加入，其摩擦係數均較單成份關節液分子來的低，其中玻尿酸混合蛋白質時，蛋白質濃度在12.5 mg/mL以下時，隨著蛋白質濃度的增加，摩擦係數沒有太大的改變，這現象可能是因為玻尿酸能鎖住蛋白質分子，防止蛋白質吸附在對摩表面上，使得摩擦係數不再上升，然而一旦蛋白質濃度大於12.5 mg/mL，其摩擦係數和單成份蛋白質溶液差不多，不再具有潤滑的效果。

關鍵字

超高分子量聚乙烯 ； 仿生潤滑液 ； 玻尿酸

並列摘要

Ultra-high molecular weight polyethylene (UHMWPE) sliding against CoCrMo ally is common in total joint implant. These polyethylene wear particles lead to osteolysis and that result in loosening and thus failure of the implant. Periprosthetic fluid presents between artificial joint surfaces and acts as a lubricant which prevents wear and reduces friction. Therefore, the constituent of synovial fluid play different roles in the joint lubrication and biomolecular interaction effect the tribological performance of artificial joint. In the present study, we have to further investigate the influence of lubricant composition on the tribological performance of artificial joints, in order to identify the roles of these lubricants in the synovial fluid.
In the study, we mix albumin,globulin,hyaluronic acid and lipid in terms of its physiological concentration for simulated synovial fluid and then add different concentration of composition to simulated synovial fluid. To study concentration of composition changes in simulated synovial fluid affect the biotribological properties of artificial articular joints. The results indicate that the friction coefficient is lowest when the concentration of hyaluronic acid is 4.5 mg/mL in the simulated synovial fluid. It is probably that hyaluronic acid can supply appropriate viscosity in the simulated synovial fluid in order to help joint lubrication. Then we further used hyaluronic acid combined with different composition to investigate biomolecular interactions on the tribological behavior of artificial joint. The results show that hyaluronic acid reduce friction coefficient compared with single composition in the all lubricant. The concentration of protein is below 12.5 mg/mL, the friction coefficient is not change along with protein increase. It is assumed that hyaluronic acid lock protein to prevent protein absorbed on bearing surface, result in friction coefficient does not arise. The concentration of protein is over 12.5 mg/mL, the friction coefficient is the same with single protein solution.

並列關鍵字

UHMWPE ； Simulated Synovial Fluid ； Hyaluronic acid

參考文獻

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