- 學位論文
發酵型玻尿酸之流變研究與溶凝膠轉換研究
Rheology Behavior and Sol-Gel Transition of Fermentated Hyaluronic Acid
摘要
玻尿酸（Hyaluronic Acid）是生化與醫療材料中常用的生物高分子，具有黏彈性（Viscoelasticity），常應用於醫療手術、整形醫學、化妝品工業等精細工業上應用，尤其是化妝品產業應用相當廣泛，一開始玻尿酸是來自於動物來源，經過生物科技與發酵工程的演進，目前多半應用菌種發酵技術來量產玻尿酸。 本實驗是使用三種不同分子量的玻尿酸粉末，依據不同濃度泡製水溶液做相關的流變性測試，主要測試方法為靜態流變法與動態流變法，靜態流變法主要測試依據相同的剪切速率（Shear rate），會呈現不同的流變行為，觀察其剪切變稀（shear-thinning）的現象；動態流變法主要測試在同一扭力（Strain）下，有不同的G’點（儲存模數）與G”點（損耗模數），本研究利用流變儀找出其溶凝膠轉換點（Sol/Gel Transition Point），探討在不同分子量的玻尿酸在不同黏度下的流變性質；同時，也測試其老化特性（Aging），因玻尿酸水溶液會因為時間而黏度微降低，研究其隨時間而改變的流變性，並觀察其是否有其他延伸結果。
並列摘要
Hyaluronic Acid (HA) is a very common biopolymer in biochemistry and medical materials due to its viscoelasticity behavior. It is widely apply on medical treatments, plastics surgery, cosmetics industry, etc., especially in cosmetics industry. In the beginning, HA was produced from animal sources. After progressing of biotechnology and fermentation technique, right now some of them are manufactured from bacterial fermentation. Our experiment was using three different molecular weight’s Hyaluronic Acid powder, and we solved them in different concentration to do the relevant rheology test. We were using Static and Dynamic Rheology test method. The method of Static Rheology is to know the rheology behavior at the same shear rate, and also to observe its shear-thinning appearance. The method of Dynamic Rheology is to know the G’(Storage Modulus) and G”(Loss Modulus) point at the same strain, so we can measure its Sol/Gel Transition Point. Due to its aging effect, we also did the rheology test with the time. We want to know its time-dependent effect and also to know its relevant results.
參考文獻
延伸閱讀
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