多孔性鈦基植體之生醫相容性研究

Research of biocompatibility on titanium-based implant with porosity

洪維彊(Wei-Chiang Hung)

指導教授 ： 歐耿良

臺北醫學大學/口腔醫學院/生醫材料暨工程研究所/碩士(2008年)

摘要

於諸多的研究顯示，鈦金屬植體於人體的生物相容性(biocompatibility)具很高的評價，其非常適合做為人體的植入物，然而鈦金屬植體之所以有這麼好的生物相容主要是與鈦金屬表面的氧化層有關。一些研究及文獻亦顯示植入生物體內材料表面的孔徑大小和細胞初始的攀附行為、增殖及分化有密切的關係，因此若能有效控制孔徑的大小將會對骨整合會有相當大助益。
本研究以電化學的陰極處理方式使鈦金屬表層形成一層氫化鈦薄膜，再以電化學陽極處理，使表面形成一層奈米多孔性的二氧化鈦(nanoporous-TiO2)結構，並以物理及化學性的分析儀器測試表面之成分、元素、膜厚、孔洞大小及結構是否符合要求，之後將對符合要求的試片在無塵室加以清洗、消毒後，即以這些鈦金屬試片進行細胞培養，經特定的時間分別對細胞的攀附，增殖作不同的測試， 並加以比較不同條件下這些測試的結果。
本研究主要是在探討鈦金屬植體表面經由複合電化學方式製作出不同的氧化層厚度及奈米孔徑大小，並以細胞實驗在不同的氧化層厚度及孔徑大小下的生長情形，此外更進一步探討奈米孔洞的表面與骨整合的癒合機制並與微奈米孔洞進行比較，此結果可對縮短植入於骨內的植體，骨整合癒合時間的機制。

關鍵字

生物相容性 ； 鈦合金 ； 奈米多孔性的二氧化鈦

並列摘要

Titanium-based alloys with excellent biocompatibility have been investigated by many researches. It is due to its passive oxide film. The surface characteristics of titanium implant, such as pore sizes/roughness, are related to initial cell behaviors and osseointegration. However, the surface design of dental implant for enhancing the rate and result of osseointegration remains unknown. The purpose of this study is to investigate the effects of the various the titanium oxide thicknesses and pore sizes/roughness on the initial attachment and proliferation of the osteoblast-like cell (MG-63) based on the above investigation, it is believed that optimal pore sizes/roughness will be promoted the osseointegration.
In this study, electrochemistry process was performed as surface treatment of titanium-based implant. Titanium hydride was formed on titanium implant surface after cathode treatment. Nanoporous titanium oxide structure was formed by anodic surface treatment. In order to realize the properties of titanium oxide film, physical and chemical properties of titanium implant with and without surface treatments. In addition, biocompatibility of titanium implant with and without surface treatments was performed by cell cultures. MTT test and cell counting is used to investigate the cell attachment and proliferation.

並列關鍵字

biocompatibility ； Titanium-based alloy ； nanoporous-TiO2

參考文獻

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