木賊對Staphylococcus simulans之抗菌活性研究

The antibacterial activity of . Equisetum hiemale L. against Staphylococcus simulans

吳筱婷(Siao-Ting Wu)

指導教授 ： 梁文俐

臺北醫學大學/藥學院/生藥學研究所/碩士(2007年)

摘要

因著人類濫用抗生素，細菌對抗生素產生抗藥性，造成很嚴重的問題。葡萄球菌對人類和動物造成普遍性的感染，Staphylococcus simulans通常在被圈養的動物(牛和豬)身上被發現，是伺機性病原菌的一種，會造成表皮炎、耳炎和乳房炎，甚至Staphylococcus simulans也被發現在成人身上，引發骨炎、關節炎甚至的敗血症。在這項研究，我們篩選了經50%乙醇萃取的八十七種傳統中藥材對產生青黴素抗藥性Staphylococcus simulans(PRSS)進行抗菌活性檢測，使用瓊脂紙錠擴散法和連續稀釋法來判斷最小抑制濃度，結果發現木賊(Equisetum hiemale L.)具有最佳抗菌活性，對PRSS的最小抑制濃度為384 ug/ml。利用分配方式分成正己烷層、乙酸乙酯層、正丁醇層和水層，發現正己烷層表現出PRSS的最小抑制濃度為256 ug/ml，所以分離正己烷層，分離出三個具有抗Staphylococcus simulans活性的化合物。經結構鑑定與抑菌活性評估，發現CP2 (saringosterol) 抑菌效果最佳，CP3 (Aristophyll-C)次之，其對PRSS的最小抑制濃度分別達32 ug/ml、64μg/ml。在抗生素與活性成分合併方面，CP2在8 μg/ml 合併penicillin 4 μg/ml及CP3在16 μg/ml 合併penicillin 16 μg/ml對PRSS有抑制生長的效果；因此CP2、CP3在抗生物質的開發上具有相當大的潛力。
關鍵字:penicillin-reststant Staphylococcus simulans;木賊; saringosterol; Aristophyll-C; PRSS

關鍵字

木賊 ； 抗菌

並列摘要

Because people abuse antibiotics, bacterial resistance to antibiotics becomes a serious global problem. Staphylococci are ubiquitous microorganisms important in human and animal infections. Staphylococcus simulans is commonly found in domesticated animals (cattle and swine), that is opportunistic pathogens, in the pathology of epidermitis, otitis and mastitis.Moreover Staphylococcus simulans was identified as the etiological agent of osteomyelitis and septic arthritis in an adult male. In this study, we screened antibacterial activities of 50% ethanolic extracts 87 traditional Chinese medicines to penicillin-resistant Staphylococcus simulans (PRSS), and used disc agar diffusion method, broth dilution method determination of minimum inhibitory concentrations (MICs). The results showed that Equisetum hiemale L. had the best antibacterial activity, and demonstrated the MICs of 384 ug/ml to PRSS. The extract were fractionated into n-Hexane, ethyl acetate, n-Butanol, H2O. The n-Hexane fraction showed MICs of 256 ug/ml to PRSS. We isolated the n-Hexane fraction to get three active compounds against staphylococcus simulans and determined the structure based on the analysis of NMR spectra. We found saringosterol and Aristophyll-C can effectively resist PRSS with MIC of 32 ug/ml and 64 ug/ml individually.Penicillin G 8 ug/ml combined CP2 4 ug/ml and Penicillin G 16 ug/ml combined CP3 16 ug/ml would be to resist PRSS. Conclude the above advantages, the CP2 and CP3 have the potential ability for the development of future antibacterial agent.
Key word: staphylococcus simulans; Equisetum hiemale L.; saringosterol; PRSS

並列關鍵字

penicillin G resistant staphylococcus simulans

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