自醱酵處理液萃取回收及分離生物製品

Recovery and Separation of Bioproducts from Treated Fermentation Liquors by Solvent Extraction

陳惠莉(Huei-Li Chen)

指導教授 ： 莊瑞鑫

元智大學/工程學院/化學工程與材料科學學系/碩士(2006年)

摘要

本論文研究利用溶劑萃取法回收與純化生物製劑，實驗共分為四部分，首先探討萃取平衡，由平衡實驗中找出最佳的分離條件。當採用批次溶劑單次萃取時，溶劑為乙酸乙酯、生物製劑濃度為2000 mg/L 、pH 8、萃取在18 h 情況下，能得到99%之萃取率。溶劑正己烷、生物製劑濃度為300 mg/L、pH 8、萃取在18 h情況下，能得到21%之萃取率。然而經過添加鹼性萃取劑Aliquat 336，正己烷為稀釋劑，自醱酵液中也可得到較佳的分離萃取效果，且萃取劑也不需前處理。
接著以平衡實驗中得到的條件，分別利用批式薄膜傳送攪拌槽及中空纖維薄膜接觸器進行萃取分離實驗。於批式薄膜傳送攪拌槽，溶劑為乙酸乙酯、生物製劑濃度為480 mg/L，得到40%之萃取率。而在溶劑為正己烷、生物製劑濃度為300 mg/L，得到15%之萃取率。由於薄膜表面積小而無法有效提升正己烷萃取效率。於中空纖維薄膜接觸器，實驗發現主要反應為吸附，吸附量達3.9 g/m2 membrane，經乙醇脫附後分析純度結果發現PVDF薄膜則是選擇性吸附。
溶劑萃取生物製劑之最終產物，經Mass spectrograms與1H NMR鑑定結果其結構無任何破壞，並且證實其結構上鍵結鹼性萃取劑而導致無乳化之性能。

關鍵字

生物製品 ； 萃取 ； 中空纖維 ； 吸附

並列摘要

In this paper, the extractive separation and recovery of bioagents from fermentation broth was studied. Batch equilibrium experiments were first conducted to find the optimal conditions for extractive separation. By performing liquid-liquid extraction with an organic solvent, we found that the best conditions were obtained when the bioagent concentration was 2000 mg/L in the aqueous phase at pH 8 and ethyl acetate was used as the solvent. The forward extraction was completed in 18 h, with 99% extraction of bioagents, but only 21% bioagent was extracted using n-hexane. On the other hand, the experimental results indicated that a better extraction efficiency could be obtained from treated fermentation broth when Aliquat 336 dissolved in n-hexane was used as the extractant.
By using two organic solvents (ethyl acetate and n-hexane) in a microporous membrane-based stirred cell, a slow extraction of bioagents from the aqueous phase was observed even at low concentration. The extraction by the solvent ethyl acetate and n-hexane was 40% and 15%, respeatively. The extraction efficiency of n-hexane could not increase because of the small membrane area. In the microporous hollow fiber membrane device, the dominant effect was the adsorption. The adsorption capacity of hydrophobic membrane for bioagent was 3.9 g/m2 membrane. Ethanol was used for the desorption of the bioagent resulting in its purification.
Bioagents isolated by solvent extraction was characterized by mass and NMR spectroscopy. The results showed that bioagents would bind with quaternary ammonium salt, leading to no emulsion formation.

並列關鍵字

Bioproduct ； extraction ； hollow fiber ； adsorption

參考文獻

Ahimou, F.; Jacques, P.; Deleu, M., Surfactin and iturin A effects on Bacillus subtilis surface hydrophobicity, Enzyme Microb. Technol., 27(2000)749-754.

Alexander, P. R.; Callahan, R. W. Liquid-liquid extraction and stripping of gold with microporous hollow fibers, J. Membr. Sci., 35(1987)57.

Alonso, A. I.; Urtiaga, A. M.; Irabien, A.; Ortiz, I. Extraction of Cr(VI) with aliquat 336 in hollow fiber contactors: mass transfer analysis and modeling, Chem. Eng. Sci., 49(1994) 901-909.

Aminabhavi, T. M.; Munnolli, R. S.; Ortego, J. D. Interaction of chlorosulfonated polyethylene geomembranes with aliphatic esters: sorption and diffusion phenomena, Waste Manage., 15(1995)69-78.

Banat, I. M., Biosurfactants production and possible uses in microbial enhanced oil recovery and oil pollution remediation: A review, Biores. Technol., 51(1995)1-12.