自醱酵處理液離子交換回收與純化生物製品

Recovery and Purification of Bioproducts from Treated Fermentation Liquors by Ion Exchange

李易書(Yi-Shu Lee)

指導教授 ： 莊瑞鑫

元智大學/工程學院/化學工程與材料科學學系/碩士(2006年)

摘要

本論文使用樹脂藉由吸附或離子交換生物製品溶液中的雜質來達到提升純度的目的，樹脂主要分為大孔徑吸附型樹脂、陰離子交換樹脂及陽離子交換樹脂。實驗分為兩個部分，首先探討各樹脂對生物製品的吸附與交換的能力。研究結果顯示陰離子交換樹脂與大孔徑吸附型樹脂均會吸附或交換生物製品，而陽離子交換樹脂在不同條件下吸附量均很少。以陰離子交換樹脂而言，交換的量與生物製品分子帶電量有關：帶負電量越高，被交換到樹脂上的量越多，分子帶電量由環境pH值控制，pH值在越接近pH6.5與pH10帶電量越多，反之相反。以大孔徑吸附型樹脂吸附效果成兩區域有不同表現，高濃度隨濃度增加飽和吸附量增加，低濃度則不符合。
其次則為純化策略的探討，利用雜質與生物製品帶電性不同與分子大小的不同來達到分離純化的目的，以控制溶液pH值與樹脂操作時間的長短尋求最佳的操作條件以達到最高的純度，以XAD7大孔徑吸附型樹脂的效果較佳，在溶液pH6.5時最為顯著由77%提升至88%，回收率則為97%，除了有不錯的純度提升能力並也有高的回收率，並將批次實驗條件放大為連續式管柱層析。

關鍵字

離子交換層析

並列摘要

The aim of this work was to increase the purity of bioproduct by adsorption or ion exchange. Three kinds of resins were used: macroporous adsorption resin, anion exchange resin, and cation exchange resin.
Initially, the adsorption ability of bioproduct to various resins was determined. The macroporous adsorption resin and anion exchange resin can adsorb/exchange bioproduct, but the cation exchange resin cannot. For anion exchange resin, the adsorbed capacity was related to the negative charge of the bioproduct: the more the charge, the more the adsorption capacity. The pH value of the solution affects the negative charge of bioproduct. The bioproduct charge was more negative when the pH was near 6.5 and 11. For macroprorous adsorption resin, the difference of the adsorption capacity of bioproduct was found in two regions, the adsorption capacity of bioproduct was higher when the initial concentration was increased. But this behavior was not been found in the low concentration region.
For the porpose of increasing purity, the adsorption time and pH value was controlled in solution. The macroporous adsorption resin XAD7 showed great ability in the enhancement of purity and recovery of the bioproduct. At pH6.5, the purity of bioproduct was increased from 77% to 88%, and the recovery of bioproduct was 97%.

並列關鍵字

Ion Exchange chromatography

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