載銀介孔生醫玻璃之合成及其抗菌形貌

Synthesis and antibacterial evaluations of silver-incorporated mesoporous bioactive glass

陳儀蓁(Yi-Jhen Chen)

指導教授 ： 石啟仁

高雄醫學大學/藥學院/香粧品學系碩士班/碩士(2013年)

摘要

根管治療是目前牙科常見的牙髓治療方式，現行的暫時性敷藥氫氧化鈣無法有效抑制根管內的次級感染細菌，如糞腸球菌。本研究以蒸發誘導自組裝法合成載銀介孔生醫玻璃，期能結合介孔生醫玻璃的生物相容性、生物活性，並具有釋放銀離子的效果，達到抑菌的作用。
載銀介孔生醫玻璃以氮氣吸/脫附曲線、掃描式電子顯微鏡和穿透式電子顯微鏡觀察材料介孔結構及表面形貌，X光繞射分析儀、傅立葉轉換紅外線光譜儀觀察粉體之晶相及鍵結，結果顯示介孔結構並不因載銀而破壞，孔徑大小約為6~7 nm，奈米銀以圓球狀結晶存在於介孔材料間，粒徑隨銀含量上升而增加，約為2~5 nm至1.5 μm。
抑菌試驗部分以大腸桿菌進行材料組成、萃取時間等抑菌試驗，並以大腸桿菌、糞腸球菌進行最小抑菌濃度（MIC）及最小殺菌濃度（MBC）及抑菌形貌評估，結果顯示：大腸桿菌之MIC為0.75 mg/ml、MBC為1 mg/ml，而糞腸球菌MIC為0.5 mg/ml、MBC為2.5 mg/ml，效果優於氫氧化鈣，其MIC為5 mg/ml。此結果顯示載銀介孔生醫玻璃具有釋放銀離子功效，作用時間1 h可有效抑菌。

關鍵字

介孔生醫玻璃 ； 銀 ； 抑菌 ； 根管治療

並列摘要

Root canal therapy is an important procedure in endodontic treatment. The current temporary dressing, calcium hydroxide, may fail to eliminate certain facultative bacteria found in the system, which might cause treatment failure. In this study, we synthesis silver-incorporated mesoporous bioactive glass (MBG-Ag). Hoping to combine the biocompatibility and bioactive of bioactive glass and releasable silver ion.
Nitrogen Adsorption/Desorption Isotherm, Transmission Electron Microscopy, Scanning Electron Microscopy, X-ray Diffraction and Fourier transform infrared spectroscopy are used to analyze MBG-Ag. The results indicate that incorporating high content of silver will tend to form ball-shape particles, and the mesopore structure (6-7 nm) won’t be affected. The time-dependent and content-dependent antibacterial activity of MBG-Ag is evaluated by using E. coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) is 0.75 mg/ml and 1 mg/ml for E. coli, 0.5 mg/ml and 2.5 mg/ml for E. faecalis, respectively. And for the golden standard of temporary dressing, calcium hydroxide, the MIC against E. faecalis is 5 mg/ml. The silver in MBG-Ag is releasable and causing the antibacterial activity after one hour exposure.

並列關鍵字

Mesoporous bioactive glass ； Silver ； Antibacteria ； Endodontics

參考文獻

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