鼷鼠感染廣東住血線蟲後，腦脊髓液凝血素濃度的變化

BALB/c鼷鼠感染廣東住血線蟲後，腦部蟲體回收數目越多，腦脊髓液中thromboxane B2的濃度也隨著升高並呈現正相關。而體外的研究，第三期及第五期幼蟲在加入花生四烯酸及cyclooxygenase抑制劑（aspirin, indomethacin, SC 560 及 nimesulide）的培養液培養後，發現第三期幼蟲在加入阿斯匹靈後第一、五及七小時thromboxane B2的產生有明顯抑制作用，而加入indomethacin後七小時也有抑制效果，但在第五期幼蟲培養液中，阿斯匹靈在七小時幾乎無抑制作用，且不論是indomethacin、SC 560還是nimesulide都無抑制效果。因此在鼷鼠的飲水中給予阿斯匹靈，結果只有抑制鼷鼠產生thromboxane B2的能力，而無法抑制蟲體產生thromboxane B2的能力，感染後十二天起，腦脊髓液thromboxane B2的濃度即顯著增加。比較BALB/c及C57BL/6J鼷鼠感染廣東住血線蟲後腦脊髓液中thromboxane濃度以及腦部出血外觀的變化，發現前者感染後thromboxane B2持續增加，但在感染後二十四天突然顯著減少，而且腦部開始明顯出血並死亡，而後者則無此種現象。因此，腦脊髓液thromboxane B2的濃度是影響鼷鼠感染廣東住血線蟲是否死亡的重要因子之一。

關鍵字

廣東住血線蟲；腦脊髓液；凝血素；環氧酵素；嗜伊紅性腦膜炎

並列摘要

Angiostrongyliasis is one of the most important zoonotic parasites in Taiwan, when non-permissive hosts infectious cause of eosinophilic meningitis and meningoencephalitis. When non-permissive hosts infecions, because the larvae migrate to the brain and spinal cord cause eosinophilia in the cerebral spinal fluid (CSF) due to non-permissive host pathological changesin the central nervous system (CNS). Thromboxane A2 is a kinds of the main products of arachidonic acid metabolism via the cyclooxygenase pathway in platelets and endothelial cells, causes vasoconstriction and platelets aggregation. Thromboxane A2 and prostaglandin I2 are known to have potent vasoactive effects on the cerebral circulation. This study measured the thromboxane concentrations by commercially available ELISA kit. In vivo, the cyclooxygenase metabolites of thromboxane changes in the cerebral spinal fluid are differently in resistant C57BL/6J mice than in susceptible BALB/c mice after infection. BALB/c mice the thromboxane concentration in the cerebral spinal fluid to the highest concentration at 12 days after infection, but C57BL/6J mice thromboxane concentration in the cerebral spinal fluid gradually increased from the beginning until to 30 days after infection. Relative of the brain tissue pathology, the thromboxane concentration in the cerebral spinal fluid of BALB/c and C57BL/6J mice were extremely interrelated. In vitro, both the third-stage larvae and fifth-stage larvae could metabolize Arachidonic acid to thromboxane. Aspirin and related non-steroidal anti-inflammatory drugs (NSAID) is inhibited to cyclooxygenase activity. To cultured the third-stage larvae and fifth-stage larvae with cyclooxygenase inhibitor like aspirin, indomethacin, SC 560 and nimesulide, individually. Aspirin could inhibit third-stage larvae at 1, 5 and 7 hours after incubation, and inhibit the fifth-stage larvae at 7 hours after incubation. Indomethacin could inhibit third-stage larvae at 7 hours after incubation. This comparative study shows relevance in thromboxane with pathology progress and opens new perspectives for understanding Angiostrongyliasis.

並列關鍵字

Angiostrongylus cantonensis ； Cerebrospinal fluid ； Thromboxane B2 ； Cyclooxygenase ； Eosinophilic meningitis

參考文獻

Alicata JE. Occurrence of Angiostrongylus cantonensis in Madagascar, Mauri. Can J Zool 1964;42:605-611

Google Scholar

Beaver PC, Rosen L. Memorandum on the first report of Angiostrongylus in man by Nomura and Lin. Am J Trop Med Hyg 1945; 13: 589-590

Google Scholar

Boullin DJ, Bunting S, Blaso WP et al. Responses of human baboon arteries to prostaglandin endoperoxides and biologically generated and synthetic prostacyclin: Their relevance to cerebral arterial spasm in man. Br J Clin Pharmacol 1979; 7: 139-147

Google Scholar

Botting R. Antipyretic therapy. Frontiers in Bioscience 2004; 9: 956-966

Google Scholar

Bruce NC. Cyclooxygenase-2 selective inhibitors: translating pharmacology into clinical utility. Cleveland clinic journal of Medicine 2002; 69: S13-S19

Google Scholar

國際替代計量

鼷鼠感染廣東住血線蟲後，腦脊髓液凝血素濃度的變化

未授權

主題瀏覽