Eosinophils Chemoattracted by Eotaxin from Cerebrospinal Fluid of Mice Infected with Angiostrongylus Cantonensis Assayed in a Microchamber

以微量滴定盤觀察感染廣東住血線蟲的老鼠腦脊髓液中的嗜酸素對嗜伊紅性球的趨化性

張一旋(Eddy Essen Chang) ； 顏全敏(Chuan-Min Yen)

《The Kaohsiung Journal of Medical Sciences》 20卷5期 (2004/05) Pp. 209-215

摘要

當非適當宿主感染廣東住血線蟲（Angiostrongylus cantonensis）時，蟲體移行到腦部之後，會出現顯著的嗜伊紅性球的增加。我們用微量滴定盤觀察BALB／c 鼠感染廣東住血線蟲二十一天後的腦脊髓液中加入不同抗嗜酸素（eotaxin），調節活化T細胞分泌表現激素 (RANTES)，巨噬細胞發炎蛋白-1α (MIP-1α)，和血小板活化因子(PAF)抗體對嗜伊紅性球趨化性的影響。我們並感染10、20、30、40 和 50 隻第三期廣東住血線蟲幼蟲，在 21 天後做蟲數密度對嗜伊紅性球趨化性的影響，以及在感染 40 隻第三期幼蟲後的第 7、14 和 21 天以研究不同時期對趨化性的影響。在腦脊髓液中加入嗜酸素或巨噬細胞發炎蛋白－1α的抗體者與未加入抗體者比較，嗜伊紅性球移行進入濾膜的數目在前者明顯減少（Student’s t test，p＜0.01，p＜0.05）。在腦脊髓液中趨化激素的活性受到感染廣東住血線蟲之密度和時間的影響。體外試驗的結果證實，當感染廣東住血線蟲時，在腦脊髓液中嗜酸素和巨噬細胞發炎蛋白－1α的產生，對嗜伊紅性球具有直接的趨化能力。

關鍵字

廣東住血線蟲 ； 趨化性 ； 嗜酸素 ； 巨噬細胞發炎蛋白-1α

並列摘要

When non-permissive hosts are infected with Angiostrongylus cantonensis, the migration of the worms to the brain and their subsequent development manifests as marked eosinophilic pleocytosis. We used microchambers to demonstrate direct eosinophil chemotactic activity by adding a variety of antibodies into cerebrospinal fluid (CSF) of BALB/c mice 21 days post-infection with A. cantonensis. The antibodies were directed to neutralize eotaxin, RANTES (regulated on activation, normal T-cells expressed and secreted), macrophage inflammatory protein (MIP)-1α, and platelet-activating factor (PAF), respectively. Eosinophil migration into the polycarbonate membrane covering CSF with anti-eotaxin or anti-MIP-1α antibodies was significantly lower than that for antibody-free CSF (Student’s t test: p ＜ 0.01, p ＜ 0.05). We also collected CSF from mice 21 days after infection with 10, 20, 30, 40, and 50 third-stage larvae (L3) respectively for dose-dependent testing, and 40 L3 at days 7, 14, and 21 after infection for time-dependent testing. Chemokine production in CSF was affected by A. cantonensis infection intensity and post-infection time. In conclusion, eotaxin and MIP-1α released in the CSF of A. cantonensis-infected mice have eosinophil chemotactic activity in this in vitro assay.

並列關鍵字

Angiostrongylus cantonensis ； chemotaxis ； eotaxin ； macrophage inflammatory protein-1α