Background Stress plays an important role in terms of maintaining healthy. The Department of Defense of the USA issued a report entitled “2002 Department of Defense survey of health-related behaviors among military personnel” in October, 2003. The report indicated that military personnel experiencing higher levels of stress of a factor of over 13.6% were more likely than those with lower levels of stress to suffer from illness, injuries, and accidents. In our country, investigation of the relationship between work-related stress with periodontal diseases and temporomandibular disorders in military personnel are limited. Hence, it is necessary to explore correlation between work stress and periodontal disease and temporomandibular disorder. Objectives The purpose of this study was to investigate the association between work stress with periodontal disease and temporomandibular disorder in different demography characteristics of military personnel. Methods There were 350 participants recruited for this study by a stratified sampling design in one military unit of southern Taiwan. Each participant completed a questionnaire and received an oral examination. The final complete data has 255 subjects with a response rate of 72.86%. The statistical analysis was conducted by JMP 5.12. Results After adjusted by factors of age groups, rank, mental health (Chinese Health Questionnaire), smoking, drinking and betel quid chewing in a logistic regression model, the result showed that people with high stress was 1.41 or 2.00 times of having community periodontal index≧2(calculus) or having loss of attachment(≧4mm), respectively, then people with less stress. However, the odds ratios were not statistically significant. Similarly, after adjusting factors of age group, rank, mental health , smoking, drinking and betel quid chewing in a logistic regression model, the result showed that people with high stress was 3.98 (p=0.0087) times of having temporomandibular disorders. People with poor mental health was 3.33 (p<0.0001) times higher than others of having temporomandibular disorders. Conclusion and suggestions Military personnel are generally younger than community population. Hence, a positive correlation was found between work stress and periodontal disease, but not in statistical significance. As for proportions of having temporomandibular disorders, we found the difference between with and without stress is 48.68% vs 22.22%, which the statistical significance was reached. While facing stress, people may perceive differently. Hence, it is important for people to have proper coping skills and to maintain good health.