氧化性迫傷於牙周治療前後之探討

Oxidative Stress in Prior and After the Treatment of Periodontal Disease

陳勝利(Sheng-Li Chen)

指導教授 ： 蔡吉政

高雄醫學大學/口腔醫學院/牙醫學研究所/碩士(2004年)

摘要

反應性氧族被認為涉及了炎性牙周疾病過程中牙周組織的破壞，其最主要的傷害性來自於氧化與抗氧化活性的不平衡，本研究的主要目的在於探討慢性牙周炎患者治療前後其牙齦溝液與唾液中兩種抗氧化劑glutathione (GSH)、glutathione peroxidase(GPx)，與脂質過氧化物lipid peroxidation(LPO)的關係。收集21位慢性牙周炎患者在治療前、後之牙齦溝液、唾液。GSH、GPx、LPO以分光光度計測量其含量。結果顯示在慢性牙周炎病患者在phase I牙周治療前後比較，治療後牙齦溝液中GPx活性及總活性上升、LPO濃度及總量減少，在唾液中，GSH濃度上升，而LPO濃度下降。本實驗顯示慢性牙周炎患者在牙周治療後其LPO降低而抗氧化劑上升的情形，此結果提供了氧化性迫傷在牙周炎之過程中扮演了相當關鍵角色的證明。

關鍵字

氧化性迫傷 ； 抗氧化劑 ； 脂質過氧化 ； 牙齦溝液 ； 唾液

並列摘要

Reactive oxygen species (ROS) are implicated in the destruction of periodontium during inflammatory periodontal diseases. The imbalance in oxidant/antioxidant activity may be the key factor to the damaging effects of ROS. This study aimed to determine the ROS oxidation products and two antioxidants, reduced glutathione (GSH) and glutathione peroxidase (GPx) levels in the gingival crevicular fluid (GCF) and saliva prior to and after the treatment of chronic periodontitis. GCF and saliva were collected from 21 patients with untreated and treated chronic periodontitis. Lipid peroxidation (LPO) products, GSH level and GPx activity were determined by spectrophotometric assay. The results (mean±SD) showed that high GPx activity and total activity, lower LPO concentration and total amount were found in the treated than untreated chronic periodontotitis patients’ GCF samples. Higher GSH concentration and lower LPO concentration also were found in the treated chronic periodontitis patients’ saliva samples. In conclusion, the decrease level of LPO with increased level of antioxidants by periodontal treament provided the evidence that oxidative stress may play a key role in the process of periodontitis.

並列關鍵字

gingival crevicular fluid ； saliva ； oxidative str

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