高氏柴胡多醣體對小鼠之免疫調節活性研究

The Study of Immunomodulatory Effect of Polysaccharide from Bupleurum kaoi in Mice

張瑞麟(Jui-Lin Chang)

指導教授 ： 顏銘宏

高雄醫學大學/藥學院/天然藥物研究所碩士班/碩士(2005年)

摘要

柴胡(Bupleuri Radix)是治療肝癌、瘧疾、間歇性發燒重要的傳統生藥之一。高氏柴胡(Bupleurum kaoi)是台灣特有種之柴胡。本研究進行高氏柴胡根部之萃取物在體外及體內的免疫調節作用。
在體內試驗中，將BALB/cByJ小鼠連續28天腹腔注射投與高氏柴胡根部熱水抽出物(BKW，300 mg/kg)、總皂素(BKS，10 mg/kg)和總多醣體(BKP，50 mg/kg、100 mg/kg)；投與後第14、21、28天犧牲老鼠，測量脾重比體重之比值，並分析在血液中CD4/CD8之比値，和血漿中免疫球蛋白的分泌量和介白質-2、干擾素-γ的濃度。在體外試驗中，使用BrdU分析小鼠脾臟的淋巴細胞增生，並分析細胞培養液中介白質-2和干擾素-γ的濃度。
體內試驗結果中指出，投與BKW，BKS和BKP後，小鼠的脾重比體重的比值每週均有明顯的增加，而血漿中的免疫球蛋白方面，BKW和BKP組別的IgG1和IgM在第14、21、28天均有顯著性上升(高於控制組)，而BKS的IgM值在第28天時為下降；此外BKP更可以促進IgG2b (在第28天)和IgG3 (在第14、21、28天)的分泌；在體外試驗方面，低濃度BKW和BKP具有小鼠脾臟淋巴細胞增生活性，並呈現劑量效應，脾臟淋巴細胞在加入低濃度BKW和BKP後，干擾素-γ濃度也明顯高於控制組，呈現劑量效應。從這些研究結果顯示，高氏柴胡可以調節免疫功能，經由提升體內免疫球蛋白，並促進體外淋巴細胞增生和干擾素-γ的分泌。

關鍵字

免疫球蛋白 ； 多醣體 ； 高氏柴胡 ； 免疫調節 ； 干擾素-γ ； 淋巴球增生

並列摘要

Bupleuri Radix (Chai-hu in Chinese and Saiko in Japanese) is one of the most important traditional Chinese crude drugs for treating hepatitis, malaria and intermittent fever. Bupleurum kaoi is one of the Bupleurum spp. families endemic in Taiwan. The immunomodulatory effects of extracts from the roots of B. kaoi were investigated in vivo and in vitro in the present study.
For in vivo study, BALB/c mice were administered with the water extract (BKW, 300mg/kg, i.p.), saponin-enriched fraction (BKS, 10mg/kg, i.p.) and polysaccharide-enriched fraction (BKP, 50mg/kg and 100 mg/kg, i.p.) for consecutive 28 days, respectively. After treatment, the ratio of spleen weight to body weight was measured, the ratio of CD4/CD8 on lymphocyte, the levels of immunoglobulins and the IL-2 and IFN-γ production in plasma were detected. For in vitro study, lymphocytes proliferation were assayed by BrdU. The IL-2 and IFN-γ production in cultured lymphocytes of BALB/c mice were measured.
As the results showed in vivo, treated with BKP significantly raised the ratio of spleen weight to body weight on days 14, 21 and 28, respectively. In the levels of immunoglobulins, the BKP and BKW of B. kaoi significantly increased the IgG1 secretion on days 14, 21 and 28 after administered to BALB/c mice in vivo. Furthermore, only BKP significantly increased the IgG2b (on day 28) and IgG3 (on days 14, 21 and 28) secretion. The IgM level was significantly increased at days 14, 21, 28 after treated with BKW and BKP; while BKS decreased the IgM level on day 21. As the results showed in vitro, low dose BKW and BKP enhanced lymphocyte proliferation and showed the increasing activity with dose-dependent. In addition, the IFN-γ production in cultured lymphocytes of BALB/c mice showed the increasing activity with dose-dependent. These results suggested that polysaccharide from B. kaoi have immunomodulatory activity via enhanced the levels of immunoglobulins (in vivo), lymphocytes proliferation and IFN-γ secretion (in vitro).

並列關鍵字

Immunomodulatory effects ； Bupleurum kaoi

參考文獻

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