利用雙波導製作即時性鋅離子螢光感測器

Fabrication of Real Time Zinc Ion Fluorescence Sensor with Double Waveguide

周哲宇(Chou, Che Yue)

指導教授 ： 洪勝富

國立清華大學/電機資訊學院/電子工程研究所/碩士(2015年)

摘要

探針式雙波導鋅離子感測器是由具有均勻光傳遞之波導效應導光板結合光纖所並行組合而成的。在導光板與光纖接合處上方塗佈薄膜水凝膠材料Poly(2-hydroxyethyl methacrylate) (Poly HEMA)並添加對鋅離子具有高選擇性之感測分子meso-2,6-Dichlorophenyltripyrrinone (TPN-Cl2)，以0.025wt%重量百分比之比例加熱均勻混合。首先，需使用激發光源波長為405nm功率為100mW之藍光二極體雷射打入導光板，利用導光板之均勻光傳遞的特性使其與光纖結合處上方之薄膜均勻接收激發光源。該薄膜接收激發光後會在波長約為622nm處發出螢光，並使用core直徑為800µm之光纖收集所散發出之螢光。此感測器可對10-6M以上濃度之鋅離子做出及時性之感測，並可在生物緩衝液Dulbecco’s modified Eagle medium(DMEM)中正常感測。探針式雙波導鋅離子感測器具有堅固結構，不易受到外力影響而造成形變導致感測異常，且製程上簡易、成本較低。利用上述概念實現具有即時性鋅離子螢光感測之效能。並期望未來能真正落實應用於生物醫學界進行活體感測，使鋅離子感測科技更向前邁進一大步。

關鍵字

波導 ； 鋅離子 ； 即時性 ； 探針式感測 ； 脊髓神經膠細胞

並列摘要

Double-waveguide zinc ion sensor combines a light guide plate that has uniformly light-transferring with an optical fiber in parallel. We coat sensing film on the junction between the light guide plate and optical fiber. The sensing film is made from Poly(2-hydroxyethyl methacrylate) which mixed with high selectivity fluorescent probe meso-2,6-Dichlorophenyltripyrrinone. The weight ratio between the two molecular is 0.025 wt%. At first, we use the blue laser diode, which the wavelength is 405 nm and the power is 100mW, as an excitation light source. By using the light guide, the laser can be transferred into the film successfully. After absorbing, the sensing film will be excited and emitting fluorescence. Eventually, the fluorescent light will be coupled into the optical fiber and collected at the other end. The double-waveguide sensor can detect instantly even the concentration of zinc ion with only 10-6M. Moreover, it can sense in Dulbecco’s modified Eagle medium(DMEM) environment. The double-waveguide sensor has a firm structure, so it's not easy to change the shape and to prevent causing abnormal sensing when it faces some force. Besides, the manufacture process of this system is simply and low cost. We use these ideas to achieve the efficacy of real-time zinc ion sensing and some applications in biomedical vivo sensing. Expecting the new kind zinc ion sensor can contribute to the biomedical sensing in vivo or ex vivo.

並列關鍵字

Waveguide ； Zinc ion ； Real-Time ； Sensing tip ； Spinal culture of Glial cell

參考文獻

「光激發螢光螢光量測的原理、架構及應用」，奈米通訊，

Drug Deliv. Lett.1,13(2011).

J. Neurosci. 31, 16076 (2011).

W. C. Chen, M. J. Tsai, and H.Cheng, Adv. Funct. Mater.23,1566(2012).

[7] H. Wang, Q. Gan, X. J. Wang, L. Xue, S. H. Liu, and H. Jiang,