利用粒線體DNA與形態分析發現新種沼蝦（Macrobrachium）

The discovery of a new species of Macrobrachium using mitochondrial DNA and morphological analysis

詹姆斯(James David Khoo)

指導教授 ： 曾晴賢

國立清華大學/生命科學院/生物資訊與結構生物研究所/碩士(2007年)

https://doi.org/10.6843/NTHU.2007.00515

摘要

A cryptic species complex containing two members of the genus Macrobrachium Bate, 1868 (Crustacea: Decapoda: Palaemonidae) has been resolved using a combination of morphology and DNA-based taxonomy.
While the character set used in morphological analysis yielded limited differentiating data, by using mtDNA sequence distances from part of the cytochrome oxidase sub unit I (COI) gene, commonly known as the “DNA barcode”, it was possible to support the inference of a new species of Macrobrachium. Based on the COI sequence data, several species groups of Macrobrachium from Taiwan could be recognised including a distinct cluster of 16 individuals of the new species. Analysis of the COI sequence showed that the majority of species groups displayed high interspecific and low intraspecific variation with all forming clusters that concur with known taxonomic boundaries.
The morphological similarity of this new species to M. latidactylus is presented here with only the shape and proportions of the terminal part of the major periopod functioning to distinguish it from the new species.
This work highlights the importance of combining traditional morphology-based taxonomy with DNA sequence data to assist in the identification of cryptic species and delineation of the genus Macrobrachium, particularly in instances of morphologically similar or damaged material.

關鍵字

mtDNA

並列摘要

A cryptic species complex containing two members of the genus Macrobrachium Bate, 1868 (Crustacea: Decapoda: Palaemonidae) has been resolved using a combination of morphology and DNA-based taxonomy.
While the character set used in morphological analysis yielded limited differentiating data, by using mtDNA sequence distances from part of the cytochrome oxidase sub unit I (COI) gene, commonly known as the “DNA barcode”, it was possible to support the inference of a new species of Macrobrachium. Based on the COI sequence data, several species groups of Macrobrachium from Taiwan could be recognised including a distinct cluster of 16 individuals of the new species. Analysis of the COI sequence showed that the majority of species groups displayed high interspecific and low intraspecific variation with all forming clusters that concur with known taxonomic boundaries.
The morphological similarity of this new species to M. latidactylus is presented here with only the shape and proportions of the terminal part of the major periopod functioning to distinguish it from the new species.
This work highlights the importance of combining traditional morphology-based taxonomy with DNA sequence data to assist in the identification of cryptic species and delineation of the genus Macrobrachium, particularly in instances of morphologically similar or damaged material.

並列關鍵字

mitochondrial tDNA ； DNA barcoding ； taxonomy

參考文獻

Armand, M. K., Ra’anan, Z., Sagi, A., & Cohen, D. 1987. Morphotypic differentiation of male Malaysian giant prawns, Macrobrachium rosenbergii. Journal of Crustacean Biology, 7 (2): 219-237.

Avise, J. C., Arnold, J., Ball, M. R., Bermingham, E., Lamb, T., Neigel, J. E., Reeb, C. A., & Saunders, N. C. 1987. The mitochondrial bridge between population genetics and systematics. Annual Review of Ecology and Systematics, 18: 489-522.

Avise, J. C., & Walker, D. 1999. Species realities and numbers in sexual vertebrates: Perspectives from an asexually transmitted genome. Proc. Natl. Acad. Sci. USA, 96: 992-995.

Bensasson, D., Zhang, D. X., Hartl, D. L., & Godfrey, M. H. 2001. Mitochondrial pseudogenes: evolution’s misplaced witnesses. Trends in Ecology & Evolution, 16 (6): 314-321.

Brown, W. M., George, M., & Wilson, A. C. 1979. Rapid evolution of animal mitochondrial DNA. Proc. Natl. Acad. Sci. USA, 76 (4): 1967-1971.