大氣低溫平面式電漿作用於纖維母細胞效應之影響

Influence of Non-thermal Atmospheric Pressure Planar-Plasma on Fibroblast

郭柏宏(BO-HONG GUO)

指導教授 ： 王明誠

中原大學/工學院/生物醫學工程研究所/碩士(2016年)

https://doi.org/10.6840/cycu201600882

摘要

介電層阻擋放電電漿目前已廣泛於醫療應用上，視為一種有效應用在傷口的治療工具，其促進皮膚細胞增殖、遷移與傷口癒合，並且對活體組織上進行殺菌功能，成為具有潛力的工具。大氣低溫電漿所產生的活性氧物種（Reactive Oxygen Species, ROS）對於細胞增殖與凋亡有息息相關，證實活性氧物種會誘導內皮細胞之成纖維生長因子（FGF）的釋放，刺激內皮細胞增生與遷移。電漿參數與纖維母細胞之間有依賴性關係，在低劑量（60秒）的電漿處理對纖維母細胞是無毒性且具有增生效果，反之高劑量（300秒）的電漿處理會有殺傷作用。本研究評估大氣低溫平面式電漿對纖維母細胞增生與活性氧物種關係性探討，利用DCFH-DA染劑標定細胞內活性氧物種含量，發現經電漿處理後細胞內活性氧物種含量上升，其對細胞增生機制有關鍵作用。在細胞內活性氧含量結果，在低劑量處理後會使細胞內活性氧含量上升且處理過後4小時會恢復至細胞原本含量濃度。以活性氧物種含量在4小時變化之機轉，評估電漿重複施打纖維母細胞的活性影響，結果證實電漿重複施打與單次施打的比較確實有活性提升趨勢，其適當的釋放細胞內活性氧物種含量可以使纖維母細胞加速增生。最後本研究成功利用平面式電漿以面積形式處理纖維母細胞，並達到促進傷口癒合效應。

關鍵字

大氣低溫電漿 ； 平面式電漿 ； 纖維母細胞 ； 傷口癒合

並列摘要

Non-thermal atmospheric pressure dielectric barrier discharge (DBD) plasma is an efficient tool recently and widely developed for medical applications such as cell proliferation, cell migration, wound healing and bacterial sterilization. Reactive oxygen species (ROS) from cell proliferation and cell apoptosis induces release of fibroblast growth factor (FGF) in endothelial cells and stimulate proliferation and migration of the endothelial cell. Cell proliferation is dependent on the exposure and dose of the plasma, where in low dose plasma (60s) was relatively non-toxic, promotes cell proliferation, while treatment at longer exposures (300s) led to cell damage. This study evaluated low temperature planar plasma in fibroblast proliferation and its relationship with reactive oxygen species. ROS interacting with the medium containing the plasma-exposed cells was measured using DCFH-DA. Plasma treatment led to increase content of intracellular ROS content, which is an important mechanism of cell proliferation. Low dose plasma treatment led to increased ROS content in cells and returned to normal level after 4 hours. Using this mechanism, cell viability was investigated with plasma treatment done in repeats. Repetitive low dose plasma treatment is better compared to a single treatment, and appropriate increase of intracellular ROS can accelerate proliferation of fibroblast. Finally, we get the optimization of non-thermal atmospheric pressure planar-plasma parameters for cell proliferation ability on fibroblast and its effect on promotion of wound healing.

並列關鍵字

Non-thermal atmospheric pressure plasma ； planar-plasma ； fibroblast ； wound healing

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