以氧化鋅奈米線指叉電極電性分析進行血型辨識

Electrical Analysis for Blood Type Identification Using ZnO interdigitated electrodes

王晨安(Chen-an Wang)

指導教授 ： 張耀仁

中原大學/工學院/機械工程研究所/碩士(2017年)

https://doi.org/10.6840/cycu201700059

摘要

本研究主要敘述以氧化鋅奈米線指叉電極晶片進行血球凝集反應的量測，由MP法(Manual Polybrene Method) 完成血液試驗的樣本，晶片主體指叉電極以微影製程製作，而為了提高晶片靈敏度，使用水熱法生長奈米線在晶片上，其中利用了正負光阻的相異性來達成可控制奈米線生長位置的目的；實驗中採用不同體積的紅血球來進行阻抗與電性的檢測，接著使用擬合的方式分析數據，比較兩種量測結果的優劣。
　　實驗結果顯示，電性量測獲得了比較好的量測結果，阻抗與電性量測在血球凝集的量測中，數值皆呈現出與血球體積成正比的線性關係，可以顯著的透過量測數值分辨出不同體積的血球，檢測最小體積量可達到0.2μl；兩種量測的主要差異在於非凝集的部分，阻抗的非凝集結果會過於靠近凝集，這導致較難以阻抗來辨別凝集與非凝集，電性量測非凝集的結果則可以明顯與凝集作出分別。

關鍵字

氧化鋅奈米線 ； 指叉電極

並列摘要

This paper describes the measurement agglutination test of erythrocytes using the interdigitated electrodes (IDE) with the Zinc oxide (ZnO) nanowire (NW). This device was fabricated by photolithography and hydrothermal method to grow the ZnO nanowire on the interdigitated electrodes. Different types of photoresists were utilized to define the location of ZnO NW selectively onto the gap of IDE. In the study, experiments were conducted based on the manual polybrene method and measured using the techniques of electrochemical impedance and electrical measurement variety volume of the erythrocytes in agglutination test sample. We use curve fitting to analysis then contrast two methods who is better.

The results show that the electrical measurements are under agglutination and non-agglutination distinguishable and more sensitive in comparison with the electrochemical impedance analyses. The measured value are discriminatiul and proportioned to the volume of agglutination. Agglutination of a trace volume of 0.2ul erythrocytes can be detected by ZnO NW-IDE, while it cannot be observed by naked eyes. To compare both measurement techniques, the measurement data were then analyzed by curve fitting. For non-agglutination, the measured value of the impedance method is close to that of non-agglutination. It is difficult to distinguish which is agglutination or non-agglutination. The electrical measurements are show the better result than the electrochemical impedance to make the identification.

並列關鍵字

Zinc oxide nanowire ； interdigitated electrodes

參考文獻

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