應用硫醇基丙基三甲氧基矽烷密封植入式 功能器電極與封裝材料孔隙之探討

Studies of Applying 3-mercaptopropyl(trimethoxysilane) on Sealing Gaps between Electrodes and Packing Material of Implantable Devices

吳宛蓁(Wan-Chen Wu)

指導教授 ： 謝明發 ； 婁世亮

中原大學/工學院/醫學工程研究所/碩士(2007年)

https://doi.org/10.6840/cycu200700479

摘要

植入式電子功能器含有微印刷電路板和電極，一般微印刷電路板需要封裝以隔離生物體組織，電極則裸露與生物組織接觸。常使用的封裝材料有聚二甲基矽氧烷和硼矽玻璃，電極多半是貴重金屬，因為封裝材料和電極材質不同，所以在封裝上將有不緻密的問題，封裝是為達到保護植入式功能器電子元件免於水氣或體液破壞之目的，確保封裝完整之重要性，是一大技術挑戰。
硫醇基丙基三甲氧基矽烷(3-mercaptopropyl)trimethoxysilane (MPTMS)經水解及縮合反應後形成網狀結構，並具有Si-O-Si以及-SH兩種官能基，有助於接合玻璃與黃金，適用為封裝材料。本研究藉溶膠-凝膠方式對MPTMS進行水解反應後，進行物理及化學特性分析、細胞毒性測試、封裝效果測試及生物相容性方面之探討。
研究結果顯示，MPTMS經溶膠-凝膠反應後具有可與玻璃及黃金鍵結的官能基，且MPTMS複合物於兩基材上皆不會發生龜裂；MPTMS複合物細胞毒性測試，結果指出，其不具細胞毒性；封裝效果測試運用二氧化碳感測器讀取數值變化量，顯示結果為MPTMS複合物具封裝效果；以組織切片研究生物相容性方面，植入大白鼠肌肉四週後測試，驗證MPTMS複合物具生物相容性。
本研究之結論為MPTMS複合物確實可與玻璃及黃金基材鍵結適用於封裝，且具有生物相容性。

關鍵字

封裝 ； 生物相容性 ； 溶膠-凝膠法 ； 硫醇基丙基三甲氧基矽烷

並列摘要

An implantable device requires hermitical packaging. In general, the device contains miniaturized electronic printed circuit boards (μ-PCB) and electrodes. The electrodes are usually designed to contact living tissues; on the other hand the μ-PCB must be properly encapsulated. Thus, encapsulation materials and the electrodes, which are likely noble metals, are partially contacted. Good candidates of the encapsulation materials include polydimethylsiloxane (PDMS) and boron silica for their biocompatible features. To ensure that the contact boundaries between the metal electrodes and the boron silica (or PDMS) are well sealed is a challenge issue. The goal of this study is to develop a biocompatible coating material for sealing the boundaries. Focuses are on (1) synthesizing a coating material to bind glass and gold; (2) evaluating the biocompatibility of the coating material; and (3) verifying the boundary sealing between a gold electrode and a boron silica tube.
In this study, (3-mercaptopropyl)trimethoxysilane (MPTMS) was synthesized through sol-gel processes. As the MPTMS sol-gel is a three-dimensional network structure and possesses functional groups of thiol and silane, covalent links of this sol-gel material with the glass tube and the gold electrode take place. This is in term that the tube and the electrode are bounded with the MPTMS sol-gel. This sealing material was through cytotoxic, biocompatible and hermetical evaluations. The results from the MTT and the agar diffusion tests confirm that the MPTMS sol-gel is a non-cytotoxic material. In addition, an animal study was performed. An MPTMS sol-gel coated gold foil was implanted into skeletal muscle tissues of a rat. Twenty eight days later the muscle tissues contacted with and adjacent to the MPTMS foil were prepared with microtomy processes and diagnosed by a microscope. The pathological diagnosis indicated no finding of inflammation showing that the MPTMS sol-gel is a biocompatible material. The MPTMS sealing effectiveness was evaluated through an MPTMS sealed gold/glass tube assembly by a home-made CO2 sensor apparatus. This study result demonstrated that no CO2 was detected. In other words, the MPTMS sol-gel can serve as a coating material to hermetically seal gaps between gold and glass.
In conclusion, the function groups of thiol and silane of the MPTMS sol-gel can be used to link with the substrates of gold and glass, respectively. Furthermore, the MPTMS sol-gel is non-cytotoxic and biocompatible; thus there is a great potential to be used to seal gold electrodes and packaging glass in the applications of implantable electronic devices.

並列關鍵字

sol-gel ； (3-mercaptopropyl)trimethoxysilane ； hermetic packaging ； bio-compatibility

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