轉換奈米粒子／蛋白暈狀體形成微氣泡對比劑方 法之研究

Study on Translating the Nanoparticle-Protein Corona into Microbubble Contrast Agents

莊文凱(Wen-Kai Chuang)

中原大學/工學院/醫學工程研究所/碩士(2011年)

https://doi.org/10.6840/cycu201100916

摘要

本研究欲建立簡單、快速之奈米粒子多功能化整合帄台，因此採用被廣泛研究也是大量
存在於人體內之材料—白蛋白，做為各種奈米粒子整合之介面。白蛋白以靜電吸附之方式，
吸附至帶有羧基官能基之奈米粒子與奈米團簇表面，形成暈狀結構而稱之粒子-白蛋白暈狀體，
為提升奈米粒子生物相容性與接枝能力之保護層與媒介。利用穿透式電子顯微鏡可明顯觀察
出各種奈米粒子帶有暈狀外型，而得以證實暈狀體之形成，且各種粒子、白蛋白與各粒子之
白蛋白暈狀體，皆使用各式光譜進行量測，並比較暈狀體合成前、後蛋白之結構、粒子之表
面電漿共振與光激發螢光等表現有無差異。本研究使用超音波乳化法將奈米粒子(或團簇)-白
蛋白暈狀體轉換為蛋白型微奈米氣泡，以醫用影像超音波驗證其帶有超音波影像對比之功用，
並且使用共軛交顯微鏡觀察氣泡之構型與光學特性，以確認氣泡之形成以及粒子嵌入氣泡與
否。研究結果可將多種或多數奈米粒子以白蛋白為基材形成氣泡，使奈米粒子更集中而提升
其特性表現，也賦予多種奈米粒子具有複合之潛力。

關鍵字

微氣泡 ； 超音波對比劑 ； 血清白蛋白 ； 螢光金奈米團簇 ； 奈米粒子

並列摘要

A general platform for nanoparticles functionalization as well as integration had been
demonstrated in the thesis. The albumin was chosen due to its’ abundance of the most amount
proteins in human serum. The albumin can spontaneously adsorb onto nanoparticles or nanoclusters
surface by electrostatic force, where nanoparticles contain plenty of carboxylic groups on their
surface. The corona shape was then formed upon the successful adsorption between nanoparticles
and albumins, resulting in a so-called complex of nanoparticle-protein corona. The proteins shell
can be improved the biocompatibility and formed as protection media for nanoparticles. The
nanoparticles within a corona-shaped shell was observed by transmission electron microscopy, and
provided the information that nanoparticle-protein corona can be formed. Different nanoparticles,
albumin and their protein corona were analyzed by spectra, TEM, surface plasma resonance effect
and photoluminescence comparing to the bared ones. Furthermore, sonochemical microemulsion
was utilized to fabricate the microbubbles from nanoparticle- protein corona. The echogenic
properties of microbubbles were tested by medical ultrasound image system, confirming if the
microbubble can increase the contrast of ultrasound image. Herein, the morphology and optical
feature of microbubbles were also observed and detected by confocal laser scanning microscopy,
demonstrating that the microbubbles can be formed with inserted nanoparticles shells. Be a
coordinator between kinds of nanoparticles, albumin and its bubble integrated different functions of
nanoparticles and created a variety of oncoming application.

並列關鍵字

ultrasound contrast agent ； fluoroescent gold nanocluster ； microbubble ； nanoparticle ； serum albumin

參考文獻

Gold and Direct Identification by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry.

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