Because the esophagus tissue spectral shape has a relationship with whether cancerous, so we used a autofluorescence microscopy system combined with a fluorescence microscopy and a spectral system to measure a lot of spectral information of the esophagus tissue. We constructed a automated control program to automate a large number of multi-point scanning, and data operations. Finally, we can use this system to scan esophagus tissue and assess a unknown spectrum whether it comes from the cancer tissue in real time by in the process of scanning has been defined by the standard spectrum of cancer and standard spectrum of normal.