非酶鎳鈷鍍層晶片之於人工唾液中的葡萄糖檢測

Detection of glucose in artificial saliva by non-enzymatic nickel-cobalt electrodeposition chip

謝慈(Tzu Hsieh)

指導教授 ： 張耀仁

中原大學/工學院/機械工程研究所/碩士(2020年)

https://doi.org/10.6840/cycu202000459

摘要

本論文旨在發展非侵入性血糖檢測，使糖尿病患者的血糖監測簡易化及避免疼痛的問題，本研究採用鎳鈷合金材料之薄膜與奈米線來修飾網版印刷電極，使用人工唾液進行非酵素型電化學檢測。鎳鈷薄膜是使用電鍍方式沉積鎳鈷合金於網版印刷電極上製成，而鎳鈷奈米線則是使用氧化鋁濾膜貼合至上述之鎳鈷薄膜，進行再一次電鍍，產生由濾膜孔洞中堆積而生長出之鎳鈷奈米線修飾網版印刷電極。隨後將鎳鈷奈米線修飾電極在不同蝕刻時間下，藉由場發射掃描式電子顯微鏡與能量散射光譜進行表徵的比較，可得最佳蝕刻時間以進行完整製程。另外針對鎳鈷薄膜與奈米線修飾電極透過循環伏安法探討於人工唾液中電催化葡萄糖的效果，兩者最低檢測限皆為25μΜ，檢測範圍為25μΜ-5mM涵蓋了生理唾液水平範圍，且皆具備良好抗干擾能力。而其中鎳鈷奈米線修飾電極在葡萄糖濃度與電流之線性相關圖中更是達到R2為0.997的決定係數， 因此此修飾電極於非酶葡萄糖檢測中具有良好的檢測能力，以及未來實際使用在生理唾液中檢測葡萄糖的展望性。

關鍵字

電化學 ； 葡萄糖 ； 人工唾液 ； 非?感測器

並列摘要

The aim of this paper is to develop a non-invasive blood glucose testing to simplify the monitoring of blood glucose in diabetic patients and to avoid the problem of pain. This study adopts a film of nickel-cobalt alloy material and nanowires, respectively, to modify the screen-printed electrodes, using artificial saliva for non-enzymatic electrochemical detection. The nickel-cobalt film is made by depositing nickel-cobalt alloy on the screen printing electrode by electroplating, while the nickel-cobalt nanowire is made by laminating aluminum oxide filter film onto the above-mentioned nickel-cobalt film, and then electroplating again to produce the nickel-cobalt nanowire decorating screen printing electrode grown from the pores of the filter film. Subsequently, the nickel-cobalt and nickel-cobalt nanowire decorated screen printing electrodes will be compared with the energy scattering spectrum by field emission scanning electron microscope at different etching times to obtain the best etching time for the complete process. In addition, the effects of electrocatalytic glucose in artificial saliva were investigated by cyclic voltammetry on Ni-Co film and nanowire modified electrodes, both with a minimum detection limit of 25μΜ and a detection range of 25μΜ-5mM covering the physiological saliva level, and both with good anti-interference ability. In the linear correlation plot between glucose concentration and current, the Ni-Co nanowire modified electrode has a determining coefficient of R2 of 0.997. Therefore, this modified electrode has good detection capability in non-enzymatic glucose detection and is promising for future practical use in the detection of glucose in physiological saliva.

並列關鍵字

electrochemistry ； glucose ； artificial saliva ； non-enzymatic sensor

參考文獻

[1] World Health Organization,“Global report on diabetes,”WHO Press, 2016.

[2] P.Saeedi, I.Petersohn, P.Salpea, B.Malanda, S.Karuranga, N.Unwin, S.Colagiuri, L.Guariguata, A.A.Motala, K.Ogurtsova, J.E.Shaw, D.Bright, R.Williams,“Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th edition,”Pratice, vol.157, pp.107843,2019.

[3] H.Y.Li, Y.L.Wu, S.T.Tu, C.M.Hwu, J.S.Liu, L.M.Chuang,“Trends of mortality in diabetic patients in Taiwan: A nationwide survey in 2005–2014,”Journal of the Formosan Medical Association, vol.118, pp.S8389, 2019.

[4] American Diabetes Association. Classification and diagnosis of diabetes . Diabetes Care 2016;39(Suppl. 1):S13–S22.

[5] American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2014;37(Suppl. 1):S81–S90.