Diabetes is the domestic top ten deadly disease and gout induced by the hyperuricemia also causes troubles with many people. Therefore, blood glucose and blood uric acid are the required investigation items for health examination. The method used for investigating the blood sugar and uric acid is by enzymatic electrochemical biosensor. This review introduces the electrochemical biosensor, its principle, and the recent development of amperometric electrochemical meter for glucose and uric acid detection, particularly, focuses on the enzyme immobilization method, the usage of redox mediator, the fabrication and materials of electrode, and their future outlook.