建構暗視野光譜系統用於單一金奈米棒之電漿共振能量轉移效應探討

Dark-field Spectroscopy of Single Gold Nanorod for Investigating Its Plasmon Resonance Energy Transfer

黃子芸(Zih-Yun Huang)

指導教授 ： 張恒雄 ； 林政鞍

中原大學/工學院/生物醫學工程研究所/碩士(2014年)

https://doi.org/10.6840/cycu201400140

摘要

本研究建構一創新暗視野光譜系統，包括暗視野顯微鏡、單光儀與影像擷取裝置，用以擷取視野中任意區塊之影像強度，不需透過光柵式光譜儀，即可得到單一金奈米粒子之散射光譜。暗視野光譜系統部份，透過單光儀輸出單一波長作為暗視野影像之激發光，透過軟體擷取特定區域之影像強度，繪製強度與時間之關係圖，再經由簡易公式將時間軸轉換為波長，即可得到該區域之散射光譜。系統建構完成後，以不同粒徑、長寬比之金奈米粒子、金奈米棒測試系統之準確度，並且進一步利用此系統探討金奈米棒作為電漿共振能量轉移效應探針之發展潛力。研究結果發現，透過暗視野光譜系統能夠即時觀測金奈米棒散射光譜消長情形，證實金奈米棒-聚亞甲藍複合體確實存在電漿共振能量轉移效應。以上研究成果可望應用於生醫感測器之開發，及相關電漿子光譜學研究。

關鍵字

亞甲藍 ； 電漿能量共振轉移 ； 暗視野顯微鏡 ； 金奈米棒 ； 散射光譜

並列摘要

This research reports a novel dark-field spectrum system including inverted dark field microscopy, monochromator, sCMOS camera and software to measure resonant Rayleigh scattering spectrum of single plasmonic nanoparticle to investigate plasmon resonance energy transfer (PRET). Monochromator as a light source, auto scan from 330nm to 690nm is achieved through graphical user interface we develop. Mono-wavelength scattered light is collected by a objective len, imaged by a sCMOS camera and analyzed by a commercial software to measure a spectrum without spectrometer. Furthermore, we report observation of plasmon quenching dips in the resonant Rayleigh scattering spectrum by plasmon resonance energy transfer from a single gold nanorod to adsorbed PolyMB.We expect that dark-field spectrum system and PRET effect of gold nanorods could have applications in real-time spectrum measure and environmental monitoring.

並列關鍵字

Plasmon resonance energy transfer ； Methylene blue ； Dark field microscopy ； Scattering spectrum ； Gold nanorods

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