羅漢果水萃物改善代謝症候群功效之探討

The Effect of Siraitia grosvenorii Water Extract on Metabolic Syndrome

黃暐皓(Wei-Hao Huang)

指導教授 ： 吳亮宜

中原大學/理學院/生物科技研究所/碩士(2019年)

https://doi.org/10.6840/cycu201900062

摘要

隨著文化的變遷及飲食生活習慣的改變，高油、高糖、高鹽飲食充斥著人們每日的三餐中，導致越來越多的人罹患代謝症候群以及代謝相關慢性疾病，如高血壓、心血管疾病、糖尿病及慢性肝病等等。因此，近年來人們開始重視健康議題，服用保健食品來預防慢性疾病的發生。羅漢果曾被報導具改善胰島素阻抗之功效，而胰島素阻抗又為代謝症候群之主要形成原因，所以本實驗目的為評估羅漢果水萃物是否具改善代謝症候群之效果。本實驗選用 Wistar 雄性大鼠餵食高油脂飼料作為誘發代謝症候群之動物模型。實驗動物共分為四組，分別為對照組 (B) :餵食一般飼料；控制組 (C) :餵食40%高油脂飼料；羅漢果水萃物低劑量組 (L) :餵食混有0.4%羅漢果水萃物之40%高油脂飼料；羅漢果水萃物高劑量組 (H) :餵食混有0.8%羅漢果水萃物之40%高油脂飼料。實驗持續8週，實驗期間分析其攝食量、體重、血壓、禁食血糖、三酸甘油酯、總膽固醇以及進行口服葡萄糖耐受性試驗 (OGTT)。實驗期結束收集其檢體分析代謝相關指標。實驗結果顯示， C 組大鼠餵食高油脂飼料後造成肥胖、高血壓、高血糖、高胰島素、三酸甘油酯與總膽固醇濃度上升及胰島素阻抗等情形，在口服葡萄糖耐受性試驗中也發現 C 組大鼠之血糖與胰島素曲線下面積皆顯著大於B組，顯示有葡萄糖耐受性不良與代謝症候群的情況。與 C 組相比，L組在三酸甘油酯及葡萄糖耐受性具有改善的現象，而H組在血壓、血糖、三酸甘油酯、總膽固醇、葡萄糖耐受性不良及胰島素阻抗皆具有改善的現象。結果顯示本實驗所使用之羅漢果水萃物具有改善高脂飲食所誘發的代謝症候群之潛力。

關鍵字

代謝症候群 ； 胰島素阻抗 ； 高油脂飲食 ； 羅漢果

並列摘要

In recent years, more and more people suffer from chronic metabolic diseases such as hypertension, diabetes, and hypertension due to the change in diet and living habit. Therefore, people begin to take nutritional supplements to prevent from the occurrence of chronic metabolic diseases. Siraitia grosvenorii has been reported to improve insulin resistance, which is the main cause of metabolic syndrome. In this study, the effects of Siraitia grosvenorii water extract (SGWE) on high fat diet induced metabolic syndrome rat were investigated.The male Wistar rats were divided into four group: basal group(B), fed with chow diet; control group (C), fed 40% high fat diet alone; low dose group (L), fed with 40% high fat diet plus 0.4% Siraitia grosvenorii water extract (SGWE); high dose group (H), fed with 40% high fat diet plus 0.8% SGWE. The study last for 8 weeks. The body weight, blood pressure, fasting plasma glucose, insulin, triglyceride, total cholesterol were measured during the experiment. The oral glucose tolerance test (OGTT) was also performed in this study. The data showed that group C had significant increases in body weight, blood pressure, fasting plasma glucose, insulin, triglyceride and total cholesterol. Group C also showed the glucose intolerance and insulin resistance. The data indicated that high fat diet could cause a cluster of disorders of metabolic syndrome. Low dose SGWE supplementation significantly ameliorated hypertriglyceridemia and impaired glucose tolerance. High dose SGWE supplementation significantly ameliorated hypertension, hyperglycemia, hypertriglyceridemia, hypercholesterolemia, glucose intolerance and insulin resistance. These findings indicated that Siraitia grosvenorii water extract supplementation is effective for improving metabolic syndrome.

並列關鍵字

metabolic syndrome ； insulin resistance ； high fat diet ； Siraitia grosvenorii

參考文獻

張俐勤，戚向陽，陳維軍，宋雲飛。(2016)。羅漢果皂苷提取物對糖尿病小鼠血糖、血脂及抗氧化作用的影響。中國藥理學通報, 22(2), 4.

張耀云（2012）。以脂肪細胞探討羅漢果中具調節血糖之活性成分。國立臺灣大學食品科技研究所碩士論文，台北市。

Abuissa, H., Jones, P. G., Marso, S. P., & O'Keefe, J. H., Jr. (2005). Angiotensin-converting enzyme inhibitors or angiotensin receptor blockers for prevention of type 2 diabetes: a meta-analysis of randomized clinical trials. J Am Coll Cardiol, 46(5), 821-826.

Akagiri, S., Naito, Y., Ichikawa, H., Mizushima, K., Takagi, T., Handa, O., . . . Yoshikawa, T. (2008). A Mouse Model of Metabolic Syndrome; Increase in Visceral Adipose Tissue Precedes the Development of Fatty Liver and Insulin Resistance in High-Fat Diet-Fed Male KK/Ta Mice. J Clin Biochem Nutr, 42(2), 150-157.

Akiyama, T., Tachibana, I., Shirohara, H., Watanabe, N., & Otsuki, M. (1996). High-fat hypercaloric diet induces obesity, glucose intolerance and hyperlipidemia in normal adult male Wistar rat. Diabetes Res Clin Pract, 31(1-3), 27-35.