二氧化鈦奈米柱免疫生物感測器應用於早期肝癌血液篩檢之研發

據行政院衛生福利部的資料顯示，肝癌長年高居所有癌症死因第二位，而世界衛生組織的資料，也顯示肝癌在全世界所有癌症中的死亡率亦高居第二位。重要的是，肝癌初期症狀並不明顯。本研究之目的為研發一種能即時測量肝癌標的物(DCP)之阻抗變化生物感測器，與其他傳統檢測方法造成的不便、花費時間過長與成本花費過高比較起來，更能達到早期預防、及時量測、與居家便利等優點，實現及早發現及早治療之目標。利用陽極處理法，製作二氧化鈦奈米柱當作二氧化鈦奈米柱生物感測器基材，最後將DCP抗體固定於感測器上製作出二氧化鈦奈米柱免疫生物感測器。經量測後發現生物感測器在高頻段942.74Hz~1.99kHz以及在低頻段3.162Hz~0.79Hz表現出良好的線性度R2 >0.9，在此頻率範圍內於高頻段呈現最佳值R2 = 0.9186，於低頻段呈現最佳值R2 = 0.9759。未來可配合讀出電路使其最終達到攜帶方便、快速即時得知量測分析結果、可居家照顧、早期預防與監測肝癌之目的。

關鍵字

肝癌；電阻抗；二氧化鈦奈米柱；生物感測器； DCP

並列摘要

According to the C.D.C statistics, liver cancer has been the second position of the top leading causes of cancer death for years, and according to WHO statistics, liver cancer is also on the second position of the top causes of cancer death among the world. Moreover, liver cancer is commonly unobservable in its early stage. The purpose of this study is to design a immunobiosensor which can immediately detect the impedance of Des-훾-carboxy prothrombin (DCP) as a biomarker of liver cancer to achieve the goal of early detection and early medical treatment in its early stage. Applying anodization, the immunobiosensor is composed of the titanium oxide nanotubes as the substrate, and the DCP antibody fixed on the sensor. Results shows that the biosensor performs good linearity in both high frequency at 942.74Hz ~ 1.99KHz and low frequency at 3.162Hz ~ 0.79Hz, with the best linearity 0.9186 at 1.55KHz in high frequency part and 0.9759 at 1.59Hz in low frequency part. Connecting with the readout circuit, the immunobiosensor will become a portable, fast and timely detection device providing the purposes of monitoring liver cancer, home care and early detection.