- 學位論文
金剛纂之水－甲醇萃取物抑制人類肝癌細胞生長初探討
Water-methanol extract from Euphorbia neriifolia L. inhibits viability of human hepatoma cells
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摘要
金剛纂屬大戟科多肉植物，原產於印度北方。傳入台灣後受到廣泛應用，成為一種民間傳統藥方，據悉作為茶湯煮沸可用於治療惡性腫瘤。本研究主要探討金剛篡茶湯之細胞毒殺效應與活性成分組成。經過MTT細胞存活測試後，發現金剛篡萃取物抑制肝癌細胞增生之效果因萃取條件而異：以熱水萃得之萃取物對HepG2細胞的毒殺能力明顯大於冷水萃取物；且熱水粗萃物可溶於甲醇之部分含有毒殺肝癌細胞之活性成分，據硫酸-乙醇顯色反應推測可能為多種黃酮類混合物。此初步結果可驗證傳統草藥之藥用效果，並且以管柱層析進一步分析其活性成分組成。實驗結果發現矽膠填充管柱以90%二氯甲烷、10%甲醇沖提所得成份有極好的抑制人類肝癌細胞效果、對HSC細胞有微弱抑制、以大鼠正常肝細胞初步測試未有明顯毒性，繼續以SPE純化可見到於365 nm紫外線下發出強烈藍色螢光成份，經由C-18管柱以HPLC分析可得到抑制人類肝癌細胞生長之成分，HPLC-MS檢測發現主要成分之分子量為631.93。
並列摘要
Euphorbia neriifolia L. is an Euphorbiaceae succulents, native to northern India. It is widely used as a traditional folk prescription after incoming Taiwan; it can be boiled as a tea for the treatment of malignant tumors. This study investigated the cytotoxic effects and active ingredients in E. neriifolia tea. MTT assays for cell viability suggested that E. neriifolia extracts inhibit cell proliferation of liver cancer as a result of extraction conditions: extract from hot water extraction possesses significantly greater cytotoxicity to HepG2 cells than the cold water extract does; the methanol-soluble portion of crude extract from hot water showed cytotoxic activity on hepatoma cells. This study verifies the traditional herbal medicinal effects, and its active ingredients were further analyzed by using column chromatography. It was found that ingredients eluted with 90% dichloromethane and 10% methanol from the silica gel column have excellent inhibitory effect on human hepatoma cells and slightly inhibitory effect on HSC cells; however, preliminary tests on normal rat liver cells did not show significant toxicity. Purified ingredients from the SPE column illuminate strongly blue fluorescence under 365 nm UV light. Determined by LC-MS, the main m/z of the peak further purified from the C-18 HPLC column with cytotoxicity is 631.93.
參考文獻
1. http://health99.hpa.gov.tw/txt/HealthyHeadlineZone/HealthyHeadlineDetai.aspx?TopIcNo=6798
2. http://www.cancer-fund.org/tc/liver-cancer.html
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延伸閱讀
- Chin, L. H. (2015). Troglitazone與Lovastatin合併使用抑制人類甲狀腺未分化癌細胞遷移之研究 [doctoral dissertation, Taipei Medical University]. Airiti Library. https://doi.org/10.6831/TMU.2015.00050
- 周鳳英、衛元耀、蔣淑娟、王天戈、開執中、戚謹文、雷永耀（1996）。以人類肝癌細胞測試硼碘化油在中子捕獲治療上之生物效應。放射治療與腫瘤學，3(4)，215-220。https://doi.org/10.6316/TRO/199603(4)215
- 陳品妤（2022）。黃晶果萃取物對人類肝癌細胞生長之影響〔碩士論文，國立屏東科技大學〕。華藝線上圖書館。https://doi.org/10.6346/NPUST202200324
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