電流式陣列型高半胱胺酸生物感測器之製作

The Fabrication of an Amperometric Homocysteine Biosensor Array

張甄娓(Jen-Wei Chang)

指導教授 ： 孫台平

共同指導教授 ： 程德勝

國立暨南國際大學/科技學院/生物醫學科技研究所/碩士(2011年)

https://doi.org/10.6837/NCNU.2011.00204

摘要

高半胱胺酸已被證實為冠狀心臟病（CHD）的重要指標之一，本研究為開發一種簡易、創新及可靠的高半胱胺酸矩陣型生物感測器（HBA）來精準地檢測高半胱胺酸，一個低成本且微小化的電流式高半胱胺酸矩陣型生物感測器是由使用網印法印刷之碳電極與使用銀-氯化銀當作參考電極所組成，該碳電極上固定了D型胺基酸氧化酶。
我們研究了HBA上工作電極數量之影響及HBA的保存期反應，HBA偵測高半胱胺酸在+300mV（相對於銀-氯化銀）時，展示出極佳地線性響應範圍（6.4-100μM）與快速反應時間（<10s），此外，HBA在八個工作電極狀況下相較於一個工作電極時，由於訊號雜訊比的增加，感測度有明顯地提升（約12倍；p<0.001），最後，HBA展現了良好的重複性（最大相對標準偏差＝3.8）及長期保存的穩定性（經過兩個月的保存後，電流響應初始值為84%）。
總之，我們研發了一種簡易、低成本、可靠與微小化高半胱胺酸矩陣型生物感測器，不論是在普通生理及病態的範圍中，都能夠精準測定血漿中之高半胱胺酸濃度。

關鍵字

高半胱胺酸 ； 生物感測器 ； 矩陣型 ； 電流式 ； 酵素

並列摘要

Homocysteine is a risk factor for coronary heart disease (CHD). This study aimed to develop a novel, simple and reliable homocysteine biosensor array (HBA) for precise determination of homocysteine. A low-cost and tiny amperometric HBA was fabricated with a D-amino acid oxidase immobilized on screen printed carbon electrodes, with silver–silver chloride (Ag/AgCl) as reference electrode. The effects of the number of working electrodes (1, 2, 4 and 8) of the HBA, and the life-span on the response of the HBA were investigated. The HBA on the detection of homocysteine at +300mV(versus Ag/AgCl) showed an excellent linear response range (6.4–100μM) and fast response time (<10 s). Moreover, the detection sensitivity of the HBA with 8 working electrodes could be significantly increased (about 12-fold; p < 0.001) as compared to that of the HBA with 1 working electrode because of the increase of signal to noise ratio. Finally, the HBA showed good repeatability (maximal relative standard deviation = 3.8%) and long-term stability (84% of its initial value of current response after 2 months of storage). In conclusion, a simple, low-cost, reliable and tiny HBA was developed being able to precisely determine plasma homocysteine concentrations in both the normal physiological and pathological ranges.

並列關鍵字

Homocysteine ； Biosensor ； Array ； Amperometric ； Enzyme

參考文獻

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