This research studies the line balancing of the blood component separation production line at the Taipei Blood Center. Work measurement and allowance time rate are used to establish standard times for each workstation in the production line and determine the cycle time. The solutions for improving the bottleneck workstations involve conducting a cost-benefit analysis to achieve the goals of balancing the workload of each workstation, increasing production capacity, and reducing costs. The results show that the improvement plans increase the output of the small bag line by 67%, and the difference ratio between the bottleneck workstations is reduced from 23% to 3%. Additionally, the output of the large bag line increases 33%, and the difference ratio decreases from 22% to 16%. Evaluating the improvement plans results in a more balanced production line where the benefits outweigh the costs.