Negativity bias, a kind of psychology effect, which indicates people perceived more influence in negative reviews than positive reviews in eWOM field. This Empirical research collect 88,250 online customer reviews in Amazon.com to conduct an OLS regression analysis and Partial Least squares (PLS) analysis. The finding reveals negativity bias does exist, which indicates negative reviews are more usefulness than positive reviews. From the perspective of sentiment analysis, the research also find that online customer reviews with more positive emotion expression are perceived less usefulness, however, online customer reviews with more negative emotion expression are perceived more usefulness. In addition, online customer reviews with review length lager are perceived more usefulness than online customer reviews with review length shorter.