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  • 學位論文

以臨界角法做共焦顯微鏡之研究

Study on Confocal Microscope by Using Critical Angle Method

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摘要


本文提出一個以臨界角法結合共焦顯微鏡的基本原理,組成一個具有高解析度及長深度量測的光學量測系統,增進原有共焦顯微鏡性能,可應用於如光學表面、生物醫學、微奈米…等三度空間影像方面的量測。本方法具有結構簡單、成本低廉、高解析度及即時量測等優點,其共焦法的縱向解析度為2nm,量測範圍為0.8μm,而臨界角法之解析度約為3nm,量測範圍為12μm。

關鍵字

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並列摘要


A new method for measuring a small displacement is presented. The principles of the measurement are based on the critical angle method and confocal microscopy. The longitudinal resolution of the confocal method is 2nm, and its measurement range is only 0.8μm. Moreover, the resolution of the critical angle method is about 3nm for NA and wavelength are 0.85 and 632.8nm, respectively, but its measurement range can reach 12μm. This optical structure could be applied to optical surface testing, bio-medical science, and nanotechnology in the future. The new technique has some merits, such as a simple and compact optical setup, high sensitivity, and high resolution.

並列關鍵字

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參考文獻


【3】Eiji Higurashi, Renshi Sawada, and Takahiro Ito, Nanometer-displacement detection of optically trapped metallic particles based on critical angle method for small force detecton, Rev. Sci. Instrum., Vol. 70, pp.3068-3073,1999.
【4】陳柏菁,共焦顯微術系統之設計與裝置,國立台灣大學電機工程學研究所碩士論文,2002.
optical phase microscopy, Opt. Lett. 23, pp. 817-819, 1998.
【7】V. Daria, C. M. Blanca, O. Nakamura, S. Kawata, and C. Saloma, Image contrast enhancement for two-photon fluorescence microscopy in a turbid medium, Appl. Opt. 37, pp.7960-7967, 1998.
microscopy through highly scattering media, Appl. Opt. 40, pp.

被引用紀錄


林彥廷(2010)。雷射掃瞄一維表面形貌之研究〔碩士論文,國立虎尾科技大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0028-1208201015164400
高培瑋(2012)。結合共光程外差干涉技術及表面電漿共振技術之表面形貌儀〔碩士論文,國立虎尾科技大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0028-0108201214290000

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