In this research, it is to fabricate bi-channel filters with a technology to fabricate Grating on Waveguide by using techniques of Holographic inference and Photolithography. In this experiment, it cooperate the technique MEMS fabrication and optical lithography to fabricate the master molding of polymer Waveguide along with techniques of microcontact printing, replica printing and soft printing, how it fabricated out the bi-channel filter is to stamp in the grating with waveguide mold into grating the of using photolithography formation. It is easily to achieve the mass production, easy fabrication and low cost by using technique of lift molding of polymeric Waveguide. In order to observe and record the results of this experiment accurately, we utilize the AFM (Atomic Force Microscopy) and SEM (Scanning Electron Microscopy).