Antrodia cinnamomea is a kind of precious medicinal mushrooms in Taiwan, But due Antrodia cinnamomea very slow grownd, so in recent years, mostly using artificial cultivationto, this study explores the matrix of the composite carbon as Antrodia cinnamomea cultured with different varieties of banana fruits ources to explore the carbon source to change the effect of Antrodia cinnamomea polysaccharides synthesis and lipase activity. The results show that the banana fruit as a medium carbon source is feasible; North banana ripening is not carbon source to replace the whole polysaccharide production and lipase activity, polysaccharides 3.5770 gmL-1 and lipase activity of 5.613 UmL-1, this feature can be used banana fruit as an alternative medium carbon source, an alternative to traditional chemical medium, as the imbalance in banana production and marketing of the corresponding program.