本研究自台灣最常見的部分發酵茶中選擇烏龍茶作為茶樣,採用散茶方式將冠突散囊菌(金花菌,Eurotium cristatum)純菌液接種至烏龍茶葉中進行發酵,以不同溫度與採樣時間探討培養出受大眾喜愛,且具生物活性的金花菌烏龍茶之最適條件。採樣期間觀察抗發炎、降血壓、抗氧化、活性成分及生菌數等各項指標指數,經分析後發現以不同發酵溫度下進行培養,在多元酚及色素物質含量上25C稍優於15C培養,而DPPH清除能力於15C與25C培養下無明顯差異,但在超氧歧化酶(SOD-like)、血管收縮素轉換酶(ACE)抑制活性、抗發炎效果方面,15C明顯優於25C培養。消費者感官品評試驗中,品評員對於15C培養出的金花菌烏龍茶整體喜愛與接受度上皆優於25C培養出的金花菌烏龍茶及發酵前之烏龍茶。綜合以上分析與品評結果,不論是在成分還是商業潛力上,15C培養出的金花菌烏龍茶皆為本次試驗中培養之最適條件。
In this study, Oolong tea, the most common fermented tea in Taiwan, was fermented with Eurotium cristatum, based on different temperatures and sampling time in order to explore and to cultivate general public favorite and acceptance, and the biological activity of the most suitable solid-state fermentation conditions for the Oolong tea. During the sampling period, the anti-inflammatory, anti -hypertensive, anti-oxidation, active ingredients and number of bacteria were investigated. The contents of polyphenol and pigments at 25C were slightly better than 15C. No significant difference in DPPH scavenging activity was found between 15C and 25C. Nevertheless, the higher SOD-Like activity, ACE inhibitory activity, and anti-inflammatory effect, were obtained under 15C cultivation. Consumer sensory evaluation test also demonstrated that the acceptance of cultivation of Oolong tea were better than 25C. Based on the above analysis and evaluation results, regardless of the composition or commercial potential, 15C are the best conditions for the cultivation of E. cristatum oolong tea.