利用電漿技術進行聚酯類表面生物相容性改質

聚乳酸聚甘醇酸(PLGA)具有生物可降解性及良好的生物相容性，以及沒有毒性，PLGA在人體可被分解為乳酸及二氧化碳而排出。PLGA高分子具有良好的機械強度，是可以由共聚合以及縮合反應，來得到不同的分子量，以藉此來調控其降解速率，可應用在生醫植入材上。軟骨硫素（chondroitin sulphate）是由D-glucuronic acid和N-acetyl galatosamine重複單位組成的硫酸化黏多醣，具有3種同形異構物。於人體中廣泛分佈在軟骨與結締組織、血管壁角膜、肌腱與皮膚中，可以增進傷口癒合的速度，且可促進關節軟骨的修復，是一種生物相容性良好的材料。但因多孔性PLGA材料之一的缺點就是表面疏水性，導致細胞不易吸附在其表面上。因此本研究針對此項缺點，PLGA多孔基材以電漿處理，以Carbodiimide當架橋劑，固定軟骨素在PLGA支架表面上。電漿處理有用Ar電漿處理以及六甲基二矽胺烷(Hexamethyldisilazane:HMDSZ)聚合沉積，前者有親水性效果(水接觸角61°-32°)，後者有疏水性效果，由SEM及FTIR圖佐證，可以發現軟骨素成功固定在PLGA支架表面，然而由纖維母細胞培養實驗，可以得知固定軟骨素的PLGA支架，其細胞生長情形較為良好。

關鍵字

軟骨素；聚酯類；電漿處理；聚乳酸

並列摘要

Porous biodegradable polymers are widely used in tissue engineering since they can provide a temporary support for cell growth and guide tissue regeneration. Poly(DL-lactide-co-glycolide) (PLGA) and PET are polyester where PLGA is popularly used in bone tissue and other biomedical applications due to its good biocompatibility and controllable biodegradability. Chondroitin-6-sulfate (CS) is the bulk of the matrix that builds vertebral discs, ligaments, cartilage and other connective tissues. Chondroitin sulfate is highly effective in recovery from injury. It can speed up healing time, reduce pain, and provide cushioning to sore, traumatized areas. In this study, plasma treatment and subsequent immobilization of Chondroitin-6-Sulfate on porous poly (DL-lactide-co-glycolide) (PLGA) scaffolds was conducted to improve the wettability, cell-, and tissue-compatibility of the scaffolds. The surface of scaffold was modified through plasma polymerization, surface graft polymerization, and their combination. After surface modification, the wettability of PLGA substrates were characterized by water adsorption ratio, the water adsorption ratio of the Ar plasma treated PLGA scaffolds increased from 201 % to 308 %. However, the water adsorption ratio of hexamethyldisilazane (HMDSZ) plasma deposited scaffolds was decreased to 177.3 %. From SEM and FTIR results, they can demonstrate the Chondroitin-6- Sulfate was immobilized on PLGA scaffolds. Cell adhesion and growth on Chondroitin-6- Sulfate was immobilized on PLGA scaffolds were more pronounced than the original one.

並列關鍵字

chondroitin sulphate ； plasma treatment ； Poly(DL-lactide-co-glycolide) ； polyester

參考文獻

[1] Min-Shyan Sheu, David M. Hudson and Ih-Houng Loh, Advanced Surface Technology, P865

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國際替代計量

利用電漿技術進行聚酯類表面生物相容性改質

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