- 學位論文
嗜鹽菌Haloferax mediterranei紅色色素之抗氧化性及褪色性反應研究
Antioxidant Properties and Decoloring Reactions of Pigments Extract from Haloferax mediterranei
摘要
本研究以嗜鹽菌Haloferax mediterranei ATCC33500為紅色色素的生產菌株，探討由菌體所萃取得之紅色色素(簡稱為hmERP)之抗氧化能力及褪色性反應。H. mediterranei嗜鹽菌的細胞膜含有紅色的菌紅素(Bacterioruberin)，是一個含有C50的類胡蘿蔔素衍生物，而已知胡蘿蔔素屬於人體所需主要抗氧化營養素，有助於降低自由基對人體的傷害。在hmERP的抗氧化性質的分析上，發現螯合亞鐵離子能力上，當hmERP於甲醇溶液中為1 mg/ml時，螯合力為35%。於清除2,2-diphenyl-1-picrylhydrazyl(DPPH)自由基的能力，在濃度為2 mg/ml時，清除力為76.3%。於還原能力方面，當hmERP濃度為4 mg/ml時，可以有明顯的將赤血鹽還原成黃血鹽之能力。於抑制共軛雙烯產生能力方面，當濃度為4 mg/ml時，其抑制力為97.2%。而hmERP對血鸚鵡的褪色性反應之研究中發現，經15個月喂食而魚身平均由5 cm長到10 cm，從外表觀察未看到外型有變型或長疣的現象，顯示hmERP對於魚之生長沒有抑制現象，同時發現魚的膚色與對照組(未餵食hmERP或餵食加麗素紅)有顯著的差異，產生類似白化現象，所以未來可進一步探討hmERP對於黑色素細胞之白化效果。
並列摘要
Haloferax mediterranei ATCC33500 as an extremely halophilic bacterium is used in this study for red pigment production. The red pigment extracted from H. mediterranei (hmERP) was tested it’s antioxidant and decoloring activity. It is known that the cell membrane of H. mediterranei contains a red pigment, bacterioruberin, which is a derivative of C-50 carotenoids. Carotenoids are well known as the major antioxidant and nutrient for human being, and help to decrease the free radicals which are harmful to human body. We have examined four of antioxidant activity of hmERP: (1) the chelating effects on ferrous ions, (2) 2,2-diphenyl-1-picrylhydrazyl (DPPH) raical scavenging activity, (3) the reducing power and (4) the antioxidant activity in linoleic acid emulsion. The chelating effect of ferrous ions was measured as 35% at hmERP concentration of 1 mg/ml. The DPPH radicals scavenging activity was measured as 76.3% at hmERP concentration of 2 mg/ml. The reduction of potassium ferricyanide to the potassium ferrocyanide was significant at hmERP concentration of 4 mg/ml. The inhibition efficiency of conjugated double bonds was 97.2% at hmERP concentration of 4 mg/ml. The decoloring reaction of hmERP was measured by feeding to Cichlasoma citrinellum synspilum. After fifteen month feeding, Cichlasoma citrinellum synspilum grew from 5 cm to 10 cm. The surface of fish had no any symptom of shape mutation or growth of warts. Comparing to the control group (without feeding hmERP), fishes by feeding hmERP had obvious decoloring skin or albinism effect. In the future we would study the possible whitening effect of hmERP on melanocytes.