嗜鹽菌Haloferax mediterranei 紅色色素之層析分離與質譜分析

The separation and analysis of Red Pigments Extracted from Haloferax mediterranei by Liquid Chromatography and Mass spectrum

許宜惠(Yi-Hui Hsu)

指導教授 ： 陳志成

大同大學/工程學院/生物工程學系所/碩士(2008年)

摘要

本實驗以嗜鹽菌Haloferax mediterranei 為紅色色素之生產菌
株，探討由菌體細胞膜萃取之紅色色素(簡稱為hmERP)的結構特徵及
分子質量。透過紫外線-可見光光譜儀(UV/VIS Spectrophotometer)吸
收光譜得知hmERP 在波長527、494、468、388、371 nm 有吸收，
其中以波長494 nm 有最大吸收值，且發現hmERP 在光照下，不同的
吸收峰皆呈現規則性的衰退；藉由紅外光光譜圖獲知，hmERP 分子
結構上具有-OH 及C=C 官能基，使hmERP 略帶極性；並經由高效液
相層析儀(HPLC)分析，設定固定相為reversed phase C18，移動相為
70%丙酮及30%水，得知hmERP 中至少含有6 種化合物，其中以滯
留時間最短的A peak 佔50.8%為主要成份。再經由化學游離法(APCI)
之液相層析質譜儀(LC/MS) 分析， 得知A peak 分子峰為
741.6(M+H)+ ， 並參考紅外光光譜圖之結果與配合菌紅素
( Bacterioruberin, 屬於C50 的類胡蘿蔔素衍生物)之基本分子結構圖推
測A peak 分子量為741，與菌紅素分子量相符。

關鍵字

嗜鹽菌 ； 質譜分析

並列摘要

Haloferax mediterranei is used in this study as an extremely
halophilic archaebacterium for red pigment (hmERP) production. In
this study, we investigate the composition, characteristic structure and
molecular weight of components in hmERP. The optical properties of
hmERP were analyzed by UV/VIS Spectrophotometer, and shown that
the absorption wavelength of hmERP are 527, 494, 468, 388 and 371
nm with maximum wavelength at 494 nm. We also found that the
absorption wavelength of hmERP decayed regularly under light
irradiation. From the infrared absorption spectrum, the molecular
structure of hmERP contain hydroxy (-OH) and C=C double bond.
After separating by reversed-phase High Performance Liquid
Chromatography (HPLC) at C18 column with 70% acetone and 30%
water as the mobile phase, the hmERP contains at least six
compounds. The molecular weight 741.6(M+H)+ of peak A (major
peak with shortest retention time) in hmERP was obtained by
LC-APCI-MS. Based on the IR spectrum, molecular structure and
molecular weigh analysis, we conclude that component in peak A of
hmERP is same as Bacterioruberin (a C50 carotenoid derivative).

並列關鍵字

Haloferax mediterranei ； Mass spectrum

參考文獻

薛顯能，2006，嗜鹽菌Haloferax mediterranei 紅色色素之抗氧化性

Bacterial sensitivity to UV light as a model for ionizing radiation

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Britton, G., 1995. Structure and properties of carotenoids in relation to

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