- 學位論文
仙草癒合組織與十種唇形花科植物之迷迭香酸、熊果酸含量、抗氧化力與酪胺酸抑制力比較研究
Comparisons of ursolic and rosmarinic acid content, antioxidant activity, tyrosinase inhibition in Mesona procumbens callus and ten kinds of Lamiaceae plants
摘要
本研究利用仙草癒合組織與十種唇形花科植物，比較其迷迭香酸 與熊果酸含量、抗氧化能力與酪胺酸抑制能力差異。在仙草癒合組 織培養累積迷迭香酸含量中，使用MS 培養基添加4 mg/l NAA 與0.5 mg/l KIN 於培養葉部及莖段培植體中，可得到最高的迷迭香酸含 量，分別為99.31±0.97 與112.98±0.05 mg/g DW，此結果與田間之彩 葉草（紅葉）之花序迷迭香酸含量相當。於相同液態培養基中添加5 g/l PVP 進行懸浮培養，可將迷迭香酸含量提升至129.61±3.24 mg/g DW。而在熊果酸含量上，而在十種唇形花科植物中，溪黃草葉部有 最高的熊果酸含量，為10.89±0.21 mg/g DW；仙草癒合組織略低於田 間仙草的含量，為0.97±0.09 mg/g DW，介於十種唇形花科植物之中， 但高於in vitro 植株。在DPPH 清除能力中，田間唇型花科植物之各 部位比較為花序>葉>莖。而仙草癒合組織與田間田間唇型花科植物在 系統濃度為100 μg/ml下，其清除能力均在90 % 以上。甲醇萃取物 不論在何種系統濃度下均較水萃取物DPPH 清除能力為強。在酪胺 酸抑制能力方面，仙草癒合組織或是田間取得之仙草，其酪胺酸 抑制能力均不明顯，在十種唇形花科植物中，彩葉草（紅葉）、廣防 風與金錢薄荷的酪胺酸抑制能力較佳。
並列摘要
The rosmarinic and ursolic acid content, antioxidant and tyrosinase inhibition activity in Mesona procumbens callus and ten kinds of Lamiaceae plants were examed in this study. The highest content of rosmarinic acid（112.98±0.05 mg/g DW）was obtained from the callus induced from stem explant of Mesona procumbens on MS media supplemented with 4 mg/l NAA or 0.5 mg/l KIN .This result was the same as the wild inflorescence of Solenostemon scutellarioides (red leaf). The contant of rosmarinic acid in cells raised to 129.61±3.24 mg/g DW when cells transfered to the same composition of liquid medium plus 5 g/l PVP. The content of ursolic acid in callus of Mesona procumbens （0.97±0.09 mg/g DW）was less than most other plants of Lamiaceae （1~10 mg/g DW）. The comparison of DPPH radical scavenging activity with different organs, inflorescence has the best radical scavenging activity, leaf second and stem third. The DPPH radical scavenging activity of methanol and water extract from callus of Mesona procumbens lower than inflorescence of Lamiaceae, and between the leaf and stem extract. Under 100 μg/mlof extract from callus Mesona procumbens and Lamiaceae plants,the DPPH radical scavenging activity were higher vi than 90 %. The average of DPPH radical scavenging activity of methanol extract was higher than water extract from all of the tested plants. The highest tyrosinase inhibition activity was expressed in Solenostemon scutellarioides（red leaf）. Neither callus nor wild plant of Mesona procumbens had obvious activity of tyrosinase inhibition.
參考文獻
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