分析六種唇型花科植物之迷迭香酸含量及其抗氧化能力

Analysis rosmarinic acid and antioxidation ability from six Labiatae plant

劉怡君(Yi-Chun Liu)

指導教授 ： 何錦玟

大同大學/工程學院/生物工程學系所/碩士(2006年)

摘要

本研究是以HPLC分析六種唇型花科植物中(左手香、紫蘇、仙草、薰衣草、迷迭香、鼠尾草)之迷迭香酸含量，總黃酮含量、及以DPPH、螯合亞鐵離子、超微弱化學冷光法比較其抗氧化能力。結果顯示：(一)六種植物的甲醇萃取物迷迭香酸含量仙草(33.89±2.18 mg/g D.W.)＞左手香(33.18±1.82 mg/g D.W.)＞紫蘇(32.91±0.25 mg/g D.W.)＞鼠尾草(22.71±0.04 mg/g D.W.)＞薰衣草(1.71±1.14 mg/g D.W.)＞迷迭香(1.40±1.75 mg/g D.W.)；(二)總黃酮含量（相當於cinnamic acid）以仙草（1.99±0.53 g/g D.W.）最高，紫蘇（1.52±0.25 g/g D.W.）次之；(三)抗氧化能力方面，六種植物的甲醇萃取物DPPH(系統濃度為10 mg /g D.W.)自由基清除能力，紫蘇和仙草都有90 ％以上；螯合亞鐵離子之能力測定以仙草最高。六種植物的水萃物以超微弱冷光法其清除超氧離子能力：紫蘇、仙草、左手香在250 μg的劑量就有50 %的清除率。羥自由基抗氧化能力：紫蘇、仙草、鼠尾草在低劑量下(7 μg)以下就有50％清除能力。

關鍵字

迷迭香酸 ； 抗氧化 ； 自由基 ； 超微弱冷光 ； 唇型花科

並列摘要

The contents of rosmarinic acid of methanol extracts from six Labiatae (Pogostemon cablin, Perilla frutescens var. acuta, Mesona chinesis Benth, Lavandula spica. L , Rosmarinus officinalis L. and Salvia officinalis L.) were analyzed by HPLC. The antioxidation abilities of extracts were analyzed by five methods (total flavonoid, DPPH radical scavenging activity, iron-chelating activity, superoxide anion activity and hydroxyl radical activity). The contents of rosmarinic acid in six Labiatae from high to low was Mesona chinesis Benth ( 33.89±2.18 mg/g D.W.)＞ Pogostemon cablin ( 33.18±1.82 mg/g D.W.) ＞ Perilla frutescens var. acuta (32.91±0.25 mg/g D.W.) ＞ Salvia officinalis L. (22.71±0.04 mg /g D.W.＞Lavandula spica. L. (1.71±1.14 mg/g D.W.)＞Rosmarinus officinalis L. (1.40±1.75 mg/g D.W.). The methanol extracts of Perilla frutescens var. acuta（1.52±0.25 g/g D.W.） and Mesona chinesis Benth（1.99±0.53 g/g D.W.） had higher amount of total flavonoid than others. In DPPH radical scavenging activity, the methanol extracts of Perilla frutescens var. acuta and Mesona chinesis Benth were all above 90% at 10 mg/ml D.W., In ferrous ion chelating effect, the methanol extracts of Mesona chinesis Benth was above 80%. In superoxide anion chelating ability, the water extracts of Pogostemon cablin, Perilla frutescens var. acuta, Mesona chinesis Benth and Saivia officinalis L were above 50% at the concentration of 250 μg D.W. . In hydroxyl radical activities, the water extracts of Perilla frutescens var. acuta, Mesona chinesis Benth and Saivia officinalis L at low dose ( 7 μg D.W.) were approached to 50%.

並列關鍵字

rosmarinic acid ； antioxidation ； free radical ； Labiatae

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