以電化學方式沉積氫氧基磷灰石薄膜 於竹炭與不鏽鋼表面

Electrochemical Deposition of Hydroxyapatite Layers on Bamboo Charcoal & Stainless Steel

施博淵(Po-Yuen Shih)

指導教授 ： 陳克紹

大同大學/工程學院/材料工程學系所/碩士(2009年)

摘要

氫氧基磷灰石(hydroxyapatite; HAp)為人體骨骼中含量較多的磷酸鈣鹽類結構，故常用以取代材之塗層，披覆於醫療植入物表面上，以增進基材之生物適應性(biocompatibility)。表面塗佈HAp方法有很多種，其中以電化學方法可容易控制鍍膜，故本研究以0.042 M 硝酸鈣(Ca(NO3)2)及0.025 M 磷酸氫二銨(NH4H2PO4)水溶液為電解液，以電化學沉積法沉積HAp於輕孔材料竹炭與對照用不鏽鋼基材上，操作參數為定電壓4~36 V、時間20~60 min.以及溫度37~80 ℃。在電化學處理過程中，因陰極區隨著OH-的產生使之pH值上升，進而促進陰極表面結晶生成磷酸鈣鹽類。性質分析方面此藉由X光繞射(X-ray diffraction, XRD)、傅利葉轉換紅外線光譜儀 (Fourier transform infrared spectrometer, FT-IR)、掃描式電子顯微鏡 (Scanning electron microscopy, SEM)、能量散佈光譜儀(Energy dispersive spectrometer, EDS)等分析儀器來分析鍍層表面型態、微結構和成分鑑定；最後在生物相容性方面則以纖維母細胞進行細胞培養，觀察細胞生長附著貼附情形以評估其細胞貼附之能力。

結果可知沉積速率隨電壓上升重量增加而緻密，亦隨時間、溫度增加而重量增加。在較高電壓高溫條件下沉積HAp較為平坦，不鏽鋼沉積速率在13.8 mg/hr．cm2到36.0 mg/hr．cm2之間，而竹炭沉積速率則在27.0 mg/hr．cm2到47.4 mg/min．cm2之間。竹炭的速率大於不繡鋼，可能是因多孔性材料表面積較大之故。纖維母細胞培養中未處理竹炭無細胞之貼附，而經電化學沉積HAp則有顯著改善細胞之貼附性，其中以高電壓(24 V)及高溫(80 ℃)沉積之試片細胞貼附量最佳。沉積之HAp鍍層將可期待於醫學植入器材的應用。

關鍵字

氫氧基磷灰石 ； 竹炭 ； 電化學沉積法

並列摘要

Hydroxyapatite (HAp) is becoming increasingly popular as a passive material on grafts such as surgical implants in the orthopedic and dental fields. Hydroxyapatite (HAp) has been extensively applied because of its superior biocompatibility and bioactivity. This study investigate into the coating of the biomedical calcium phosphate on the bamboo charcoal and stainless steel substrate in a 0.042 M (Ca(NO3)2) and 0.025 M (NH4H2PO4) solution under 4 ~ 36 V, 37 ~ 80 ℃ for 30 min to 1 hr. Homogeneous coatings were attained by electrochemical method. This coating technique could be a good control method for the thickness, morphology, crystallinity, and stoichiometry of the deposits. Hydroxide groups on the substrate surface favor the chemical bonding with calcium and phosphate ions to produce HAp coating on the cathode surface.

X-ray diffraction (XRD), Fourier transform infrared spectrometer (FT-IR), scanning electron microscopy (SEM) and energy dispersive spectrometer (EDS) were used to observe the surface morphology and identify phase of the coating layer. As a result, the increasing deposition rate of HAp followed increasing temperature, time and voltage. HAp layer becomes smoother at high temperature and high voltage. The deposition rate of HAp on stainless steel is between 13.8 mg/hr．cm2 to 36.0 mg/hr．cm2. On the other hand, on bamboo charcoal the deposition rate of HAp is 27.0 mg/hr．cm2 to 47.0 mg/hr．cm2. The rate of bamboo charcoal is better than stainless steel, the reason could be that bamboo is a porous material, and the surface area is bigger. Finally in this study Fibroblast (CCD-966SK) cell line was employed for cell attachment test. After incubating for 3 days the cells are observed great attachment and growth on high temperature and voltage coated HAp layer. This process may be application in medical implant device.

並列關鍵字

bamboo charcoal ； electrochemical method ； Hydroxyapatite

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