- 學位論文
高劑量果糖轉移酶對蔗糖的催化反應
Catalytic Reaction of High Dose Fructosyltransferase on Sucrose
摘要
本研究探討用不同劑量的日本麴菌的果糖轉移酶催化蔗糖。一般生產果寡糖時使用低劑量酵素的產物是蔗果三醣(GF2)、蔗果四醣(GF3)以及蔗果五醣(GF4)，另外還有葡萄糖(G)副產物。使用高劑量的酵素時，發現除了前述的G、GF2、GF3、GF4以外，尚有F、F2、 F3、F4以及GF5等醣類。 果糖轉移酶催化反應是使用100 mL 的50%w/v的蔗糖(GF)，溶於0.1M的檸檬酸鈉緩衝液 (pH5.5)，於30℃水浴中進行33小時，然後用高效液相層析法分析反應液中的醣類。使用225 U 果糖轉移酶催化反應24小時候，出現GF4以及F、G、GF2、GF3等一般產物。使用450 U 酵素催化反應24小時候，出現F3以及F、G、GF2、GF3、GF4等產物。使用900 U酵素催化反應24小時候，出現F2、F3、F4以及F、G、GF2、GF3、GF4等產物。使用1800 U酵素催化反應24小時候，更額外出現了新產物GF5。反應過程中，G與F3、F4、GF4、GF5隨催化時間增加而增加，GF隨時間增加而減少，但從6小時後趨於穩定。當酵素劑量增加到3600 U和7200 U時，其中G2、F2、F3、F4的濃度都有顯著提升，而GF5的濃度在酵素量7200 U反應17小時達到最高。
並列摘要
In this work catalytic reactions were investigated under sucrose being catalyzed by various doses of fructosyltransferase (Ftase) from Aspergillus japonicus. Generally, for the production of fructooligosaccharides, low dose of Ftase was used on sucrose, yielding 1-kestose (GF2), nystose (GF3), fructosyl nystose (GF4,) and the byproduct glucose (G). However, when high dose of Ftase was used, not only G, GF2, GF3 and GF4, as previously described, but also fructose (F), F2, F3, F4, F5, GF5 were produced. The enzyme reactions were carried out in 100mL of 50%w/v sucrose in sodium citrate buffer (pH 5.5) at 30°C for 33 hrs and sugars in the reaction mixtures were analyzed by HPLC. After 24 hrs of reaction, F, G, GF2, GF3 and GF4 were produced, when 225U of Ftase was used. F, G, GF2, GF3, GF4 and F3 were produced, when 450U of Ftase was used. F, G, GF2, GF3, GF4 and in addition F2 and F3 were produced, when 900U of Ftase was used. Furthermore, F, F2, F3, G, GF2, GF3, GF4 and GF5, a new product, were produced, when 1800U of Ftase was used. During the reaction, G, F3, F4, GF4 and GF5 increased with reaction time. At the first 6 hrs, GF decreased with time and then kept in a constant level. When Ftase up to 3600U and 7200U were used, among various sugars, G2, F2, F3 and F4 increased significantly. GF5 peaked at 17 hrs, when 7200 U of Ftase was used.
參考文獻
延伸閱讀
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