利用電漿處理及UV光接枝聚合改善固定軟骨素於純鈦表面之研究

鈦合金在植牙材與骨植入材上已經有相當廣泛的應用，而增進植牙材料表面與患部癒合為重要的議題，因此在純鈦表面上固定一層類細胞外基質之結構期望增進細胞的生長。但是金屬上沒有可固定生物分子之結構故本研究表面引入界面膜利用電漿處理即六甲基二矽氨烷( Hexamethyldisilazane，HMDSZ )電漿在植體表面沉積一層有機矽薄膜，可在其表面固定其他機能性高分子。又因有機矽膜為疏水性薄膜水接觸角約為90°，此疏水性質能改善抗蝕性。抗蝕電位經HMDSZ處理後從-0.41~ -0.07V，在HMDSZ 60W 60mtorr 10min 有最佳抗蝕性腐蝕電位約在-0.07 V。另外再使用氧氣(O2)電漿活化表面產生自由基，即可UV-light將丙烯酸(Acrylic Acid)單體接枝在電漿膜表面，使表面呈親水性且水接觸角約50°，此丙烯酸表面可使用EDC/NHS化學交聯將幾丁聚醣與軟骨素固定，固定率可達每平方公分3.83克。大鼠骨隨幹細胞培養結果顯示，未處理試片及氧氣親水化後之表面生長情形良好，細胞生長情形較佳，而疏水性之PD-HMDSZ表面及經AAc接枝以及固定幾丁聚醣和軟骨素之細胞貼附情形較差。

關鍵字

接枝；軟骨素；電漿

並列摘要

Titanium Alloy had been wide used in implants. Improve implant surface healing with wound is important discussion. The study is immbolised extracellular bone matrix (ECM) on the Titanium surface excepting that will improve cells growth. The study used plasma treatment of Hexamethyldisilazane ( HMDSZ) that treated on imapnt surface deposition organic silicon film.This organic silicon film contains carbon, nitrogen, oxygen functional groups that could be modified other functional groups. The organic silicon flim is hydrophobic that water contact angle is about 90°. This hydrophobic thin film could improve corrosion resistance. The corrosion volts after HMDSZ plasma treatment is about -0.41~-0.07V.HMDSZ plasma treated 60W 60mtorr 10min has the best corrosion volts is about -0.07V. Surface of oxygen plasma treated the HMDSZ thin film that generated radical.Surface of UV-light grafted Acrylic Acid (AAc) on the deposition film. The surface is hydrophilic. The water contact angle is about 50°. Surface of EDC/NHS cross linked chitosan and chondroitin on the surface.The graft density is about 3.83mg/cm2. Surface of the rats bone marrow stem cell culture, the untreated Titanium cell culture is well. O2 plasma treatmed PD-HMDSZ cell culture is well. After graft AAc and immobilise chitosan with chondroitin cell culture is not well.

並列關鍵字

graft ； chondroitin ； plasma

參考文獻

[2] Moroni A, Heikkila J, Magyar G, Toksvig-Larsen S, “Giannini S.Fixation Strength and Pin Tract Infection of Hydroxyapatite-Coatedtapered pins.” Clin Orthop 2001;388:209–17.

[3] Sande’n B, Olerud C, Petre’n-Mallmin M, Larsson S. “Hydroxyapatite coating improves fixation of pedicle screws. A clinical study.” J Bone Jt Surg Br 2002;84-B(3):387–91.

[4] Shepperd J.A, Apthorp H. “A contemporary snapshot of the use of hydroxyapatite coating in orthopaedic surgery.” J Bone Jt Surg Br 2005;87(8):1046–9.

[5] Augat P, Claes L, Hanselmann KF, Suger G, Fleischmann W. “Increase of stability in external fracture fixation by hydroxyapatitecoated bone screws.” J Appl Biomater 1995;6(2):99–104.

[6] Ruoslahti E, Yamaguchi Y. “Proteoglycans as modulators of growth factor activities. ” Cell 1991;64(5):867–9.

國際替代計量

利用電漿處理及UV光接枝聚合改善固定軟骨素於純鈦表面之研究

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